Go-ChIP-Grade™ Purified anti-Oct4 (Oct3) Antibody

Pricing & Availability
Clone
3A2A20 (See other available formats)
Other Names
POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3A2A20_GoChIP_OCT4_Antibody_1_052118
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 NCCIT cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Oct4 (Oct3) Antibody (clone 3A2A20, Cat. No. 653713) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human SOX2 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 3A2A20_GoChIP_OCT4_Antibody_1_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 NCCIT cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Oct4 (Oct3) Antibody (clone 3A2A20, Cat. No. 653713) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human SOX2 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 3A2A20_GoChIP_OCT4_Antibody_2_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 NCCIT cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Oct4 (Oct3) Antibody (clone 3A2A20, Cat. No. 653713) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human OCT4 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 3A2A20_Purified_OCT4_Antibody_1_WB_051314
    Total lysates (15 µg protein) from NTERA2 (lane 1), HeLa (lane 2) and NF-1 (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-Oct4(Oct3) Antibody, clone 3A2A20 (upper), or 1:2000 diluted anti-GAPDH Antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-Oct4 Antibody or 1:3000 diluted Donkey anti-Rabbit-IgG secondary antibody conjugated to HRP for anti-GAPDH Antibody. Lane M: Molecular weight ladder.
  • 3A2A20_Purified_OCT4_Antibody_2_ICC_080219
    NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti-Oct4 (Oct3) (clone 3A2A20) and followed by DyLight™ 594 goat anti-mouse IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
653713 25 µg $75
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653714 100 µg $235
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Description

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Oct4 recombinant protein (1-141aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP. For ChIP, the suggested use of this reagent is 1:50 to 1:100 by volume. For Western blotting, the suggested working dilution(s): Use 0.5 µg/ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration of 2.5 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3A2A20 has been found to weakly cross react with mouse.

RRID
AB_2716040 (BioLegend Cat. No. 653713)
AB_2716041 (BioLegend Cat. No. 653714)

Antigen Details

Structure
Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution

Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction
SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area
Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271.

Gene ID
5460 View all products for this Gene ID
UniProt
View information about Oct4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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