- Other Names
- Multiplex assay, Flow cytometry, Insulin Like Growth Factor Binding Protein, IGF-Binding Protein, IGFBP, Flex set, CBA, LEGENDplex
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The LEGENDplex™ Human IGFBP Standard can be used together with the following Mix and Match Capture Beads and Human IGFBP Panel Detection Antibodies to offer flexible customization within the panel (7-plex):
740471 (LEGENDplex™ Human IGFBP Panel Detection Antibodies)
740472 (LEGENDplex™ Human IGFBP-7 Capture Bead A4, 13X)
740473 (LEGENDplex™ Human IGFBP-3 Capture Bead A5, 13X)
740474 (LEGENDplex™ Human IGFBP-6 Capture Bead A8, 13X)
740475 (LEGENDplex™ Human IGFBP-2 Capture Bead B2, 13X)
740476 (LEGENDplex™ Human IGFBP-4 Capture Bead B4, 13X)
740477 (LEGENDplex™ Human IGFBP-1 Capture Bead B5, 13X)
740478 (LEGENDplex™ Human IGFALS Capture Bead B9, 13X)
740377 or 740379 (Filter Plate or V-bottom Plate for LEGENDplex™ Assay)
740374 (LEGENDplex™ Buffer Set C)
The assembled Human IGFBP Mix and Match Panel is a multiplex bead-based assay panel that uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of up to 7 IGFBPs: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-7, and IGFALS. This assay panel provides broader dynamic ranges than traditional ELISA methods, and has been validated for use with serum, plasma, cell culture supernatant, and other biological fluids samples.
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.