- Other Names
- Human M1/M2 Macrophage Panel (10-plex), Multiplex, flow cytometry, Macrophage, Microglia, M1, M2, LEGENDplex, IL-12p70, TNF-ž€, IL-6, IL-10, IL-1ž, TARC, IL-1RA, IL-12p40, IL-23, IP-10
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Macrophages are derived from the differentiation of monocytes in response to an infection or tissue damage. Their primary function is to recognize, engulf, and destroy pathogens, dying or dead cells and cellular debris. Macrophages are also professional antigen presenting cells that play a crucial role in modulating the host response, secreting an array of cytokines which aid in host defense, tissue repair, and immunoregulation.
The LEGENDplex™ Human M1/M2 Macrophage Panel (10-plex) is a bead-based multiplex assay panel, which uses fluorescence-encoded beads suitable for use on various flow cytometers. This Panel focuses on 10 key targets involved in M1 and M2 macrophage biology: TNF-α IL-1β, IL-1RA, IL-6, IL-10, IL-12p40, IL-12p70, IL-23, CCL17 (TARC), and CXCL10 (IP-10). It provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. This panel has been validated for use on cell culture supernatant, serum, and plasma samples.Product Details
- Kit Contents
- Setup Beads 1: FITC Beads (1 ml)
- Setup Beads 2: PE Beads (1 ml)
- Setup Beads 3: Raw Beads (2 ml)
- Capture Beads (varies, see manual)
- Detection Antibodies (3.5 ml)
- Standard Cocktail, Lyophilized (1 vial)
- SA-PE (3.5 ml)
- Matrix B1, Lyophilized (1 vial)
- Assay Buffer (25 ml)
- Wash Buffer, 20X (25 ml)
- Plate Sealers (4 sheets)
- Data Analysis Software Dongle
- V-bottom Plate
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
- Materials Not Included
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Biology Area
- Immunology, Innate Immunity, Neuroscience, Neuroinflammation
- Molecular Family
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.