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MojoSort™ Mouse CD3 Selection Kit

Pricing & Availability
Regulatory Status
RUO
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Product Citations
publications
Mouse_CD3_selection_kit_1_010218
A single cell suspension from C57BL/6 mouse splenocytes was prepared for positive selection of CD3+ cells using the MojoSort™ Mouse CD3 selection Kit. Cells were stained with anti-mouse CD3 (clone 145-2C11) PE. Dead cells was excluded by 7-AAD.
  • Mouse_CD3_selection_kit_1_010218
    A single cell suspension from C57BL/6 mouse splenocytes was prepared for positive selection of CD3+ cells using the MojoSort™ Mouse CD3 selection Kit. Cells were stained with anti-mouse CD3 (clone 145-2C11) PE. Dead cells was excluded by 7-AAD.
  • Mouse_CD3_selection_kit_2_010218
Cat # Size Price Quantity Check Availability Save
480099 20 tests $198
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480100 200 tests $594
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Description

Mouse CD3+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse CD3 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD3+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Clone 17A2 recognizes ε/γ (but not ε/δ) of the CD3 complex. It can induce T cell activation.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat. No. 480099:

  • 1 vial containing 200 µl of Biotin anti-mouse CD3 antibody (clone 17A2)
  • 1 vial containing 200 µl Streptavidin Nanobeads

For Cat. No. 480100:

  • 2 vials containing 1ml each of Biotin anti-mouse CD3 antibody (clone 17A2)
  • 2 vials containing 1ml each of Streptavidin Nanobeads

Product Details

Verified Reactivity
Mouse
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibody was purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin Nanobeads: protein-coated magnetic nanobeads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of Biotin anti-mouse CD3 antibody for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of mouse CD3+ cells from lymphoid tissue.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 4X

Product Citations
  1. Wang YM, et al. 2022. Oncoimmunology. 11:2034257. PubMed

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Gene ID
NA
UniProt
View information about CD3 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 05/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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