- HCD57 (See other available formats)
- Other Names
- HNK-1, NK-1, Leu-7
- Mouse IgM, κ
- Ave. Rating
- 2 reviews
- Product Citations
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CD57, also known as HNK-1, NK-1, and Leu-7, is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The HCD57 antibody has been shown to recognize human CD57 and to be useful for flow cytometry.
(PubMed link indicates BioLegend citation)
- Kaplan RC, et al. 2011. J. Infect Dis. 10:76. PubMed
- Lutz CT, et al. 2011. J. Immunol. 186:4590. PubMed
- Leeansyah E, et al. 2013. Blood. 121:1124. PubMed
- Simmons R, et al. 2013. J Virol. 87:3087. PubMed
- Kurktschiev PD, et al. 2014. J Exp Med. 211:2047. PubMed
- Perreau M, et al. 2014. J Exp Med. 211:2033. PubMed
- Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
- Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
- Product Citations
AB_2063198 (BioLegend Cat. No. 322315)
AB_2063197 (BioLegend Cat. No. 322316)
- Oligosaccharide antigenic determinant present on a variety of proteins, lipids, and chrondroitin sulfate proteoglycans, 100-115 kD. The antigen is conserved across species.
- Expressed on 12-20% of peripheral blood lymphocytes including a subset of NK cells and a subset of CD8+ T cells. Also expressed on receptors on neural cells and striated muscle. Not expressed on red cells, granulocytes, monocytes, and platelets.
- Unknown function, may be involved in cell-matrix interactions.
- Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.
- Cell Type
- Lymphocytes, NK cells, T cells
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
- Gene ID
- 27087 View all products for this Gene ID
- View information about CD57 on UniProt.org
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