PE anti-mouse CD194 (CCR4) Antibody

Pricing & Availability
Clone
2G12 (See other available formats)
Other Names
CC CKR-4, CHEMR1, Cmkbr4, LESTR, MGC151418, Sdf1r
Isotype
Armenian Hamster IgG
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Product Citations
publications
2G12_PE_102108.jpg
Hyper-immunized BALB/c splenocytes stained with GK1.5 (CD4) APC and 2G12 PE
  • 2G12_PE_102108.jpg
    Hyper-immunized BALB/c splenocytes stained with GK1.5 (CD4) APC and 2G12 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
131203 25 µg $120
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131204 100 µg $280
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Description

Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Saito K, et al. 2008. J. Immunol. 181:6889. PubMed
  2. Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed
  3. 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed
  4. Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
  5. Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed.
  6. Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.
Product Citations
  1. Lee H, et al. 2010. J Immunol. 185:6698. PubMed
  2. Moriwaki A, et al. 2011. Am J Respir Cell Mol Biol. 44:448. PubMed
  3. Ohsugi T, Kumasaka T 2011. PLoS One. 6:e18518. PubMed
  4. Liu Y, et al. 2013. Cancer Res. 73:550. PubMed
  5. Loebbermann J, et al. 2013. Proc Natl Acad Sci U S A. 110:2987. PubMed
  6. Suga H, et al. 2013. J Immunol. 190:6059. PubMed
  7. Honjo A, et al. 2013. Respir Investig. 51:241. PubMed
  8. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  9. Meadows D, et al. 2015. PLoS One. 10: 0143738. PubMed
  10. Wiesner D, et al. 2016. J Immunol. 196: 365 - 374. PubMed
  11. Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed
  12. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  13. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  14. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  15. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
RRID
AB_1236369 (BioLegend Cat. No. 131203)
AB_1236367 (BioLegend Cat. No. 131204)

Antigen Details

Structure
GPCR, chemokine receptor, seven transmembrane receptor
Distribution

Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs

Function
CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
Interaction
Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
Ligand/Receptor
CCl17 and CCL22
Cell Type
T cells, Macrophages, Platelets, Basophils, Th2, Tregs, Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171.
2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30.
3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901.
4. Clark RA and Kupper TS. 2006. Blood 109:194.
5. Lee I, et al. 2005. J. Exp. Med. 201:1037.

Gene ID
12773 View all products for this Gene ID
UniProt
View information about CD194 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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