PE anti-mouse CD309 (VEGFR2, Flk-1) Antibody

Pricing & Availability
Clone
Avas12 (See other available formats)
Other Names
Fetal liver kinase 1 (Flk-1), VEGF receptor-2, VEGF-R2, Ly-73, KDR
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Avas12_PE_122209
Endothelial bEnd.3 cells stained with Avas 12 PE
  • Avas12_PE_122209
    Endothelial bEnd.3 cells stained with Avas 12 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
136403 50 µg $140
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136404 200 µg $340
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Description

CD309 is also known as vascular endothelial growth factor receptor 2 (VEGFR2) and fetal liver kinase-1 (Flk-1). CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. CD309 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. It's a receptor for VEGF or VEGFC, and plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine Flk1 fused to hIgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB)
  2. Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC)
Product Citations
  1. Gong H, et al. 2016. Stem Cell Reports. 7:1050-1058. PubMed
RRID
AB_1967093 (BioLegend Cat. No. 136403)
AB_1967124 (BioLegend Cat. No. 136404)

Antigen Details

Structure
A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily
Distribution
Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle
Function
Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability
Ligand/Receptor
Vascular endothelial growth factor (VEGF)
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kaburn N, et al. 1997. Development. 124:2039
2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111
3. Nishikawa SI, et al. 1998. Immunity 8 (6):761
4. Shalaby F, et al. 1997. Cell. 89 (6):981

Gene ID
16542 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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