PE anti-mouse/rat CD81 Antibody

Pricing & Availability
Clone
Eat-2 (See other available formats)
Other Names
TAPA-1
Isotype
Armenian Hamster IgG
Ave. Rating
1 reviews
Product Citations
publications
EAT-2
C57BL/6 mouse splenocytes stained with Eat-2 PE
  • EAT-2
    C57BL/6 mouse splenocytes stained with Eat-2 PE
  • EAT-2 Rat
    C57BL/6 mouse splenocytes stained with Eat-2 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
104905 50 µg $105
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104906 200 µg $290
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Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1. CD81 is expressed on T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, and fibroblasts. CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CD81+ mouse B lymphoma 38C13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The Eat-2 antibody reacts with mouse and rat CD81. Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 104908).

Application References

(PubMed link indicates BioLegend citation)
  1. Maecker HT, et al. 2000. Hybridoma 19:15. (IP, WB, Stim)
  2. Clark KL, et al. 2001. J. Immunol. 167:5115. (IP, WB, Activ)
  3. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200. PubMed
  4. Castro-Seoane R, et al. 2012. PLoS Pathog. 8:1002538. PubMed
Product Citations
  1. Rahman M, et al. 2014. Diabetes. 63:1008. PubMed
  2. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  3. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  4. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
RRID
AB_2076267 (BioLegend Cat. No. 104905)
AB_2076266 (BioLegend Cat. No. 104906)

Antigen Details

Structure
TM4SF tetraspanin, 26 kD
Distribution
T cells and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts
Function
Regulates cell growth
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Levy S, et al. 1998. Annu. Rev. Immunol. 16:89.
3. Maeker HT, et al. 1997. FASEB J. 11:428.
4. Boismenu R, et al. 1996. Science 271:198.

Gene ID
12520 View all products for this Gene ID 25621 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org

Reviews

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Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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