Propidium Iodide Solution

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Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

Product Details
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421301 2 ml $45.00
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Technical data sheet

Product Details

Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
0.5 mg/ml
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.

FC - Quality tested

Recommended Usage

The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with care.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.

Application References

(PubMed link indicates BioLegend citation)

1. Vermes I, et al.1995. J. Immunol. Methods 184:39.
2. Darzynkiewicz Z, et al. 1992. Cytometry 13(8):795.
3. Douglas RS, et al. 1995. J. Immunol. Methods 188:219.
4. Sakimoto I, et al. 2006. Cancer Research 66:2287.PubMed
5. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
6. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
7. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
8. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
9. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
10. Ewald SE, et al. 2014. Infect Immun. 82:460. PubMed
11. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
12. Kong S, et al. 2014. J Immunol. 193:5515. PubMed

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Related FAQs

Why washing is not recommended post 7AAD or PI addition for viability check?
These dyes bind in equilibrium with DNA. Therefore external dye concentration must be maintained during analysis and the dye should not be washed out.
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