Purified anti-FITC Maxpar Ready

Pricing & Availability

Clone: FIT-22 (See other available formats)
Regulatory Status: RUO
Other Names: Fluorescein isothiocyanate
Isotype: Mouse IgG1, λ
Ave. Rating: Submit a Review
Product Citations: publications

Human PBMC stained with FITC-anti-CD3 (UCHT1) then detected with 174Yb anti-FITC (FIT-22). Plotted against 154Sm-anti-CD45 (HI30). Data provided by DVS Sciences.

Compare all formats

Cat #

Size

Price

Quantity Check Availability

Save

408305

100 µg

$141

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).

Product Details

Technical data sheet

Product Details

Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: FITC conjugated mouse IgG.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563769 (BioLegend Cat. No. 408305)

Antigen Details

Gene ID: NA
UniProt: View information about FITC on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All FITC Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-FITC	FIT-22	FC
Biotin anti-FITC	FIT-22	FC
Purified anti-FITC (Maxpar® Ready)	FIT-22	FC,CyTOF®
APC anti-FITC	FIT-22	FC
PE anti-FITC	FIT-22	FC
TotalSeq™-C0988 anti-FITC	FIT-22	PG
TotalSeq™-A0988 anti-FITC	FIT-22	PG
TotalSeq™-B0988 anti-FITC	FIT-22	PG

Certificate of Analysis

Customers Also Purchased

Ultra-LEAF™ Purified anti-mouse CD28

Clone: 37.51

Purified anti-phycoerythrin (PE) (Maxpar® Ready)

Clone: PE001

Recombinant Human M-CSF (carrier-free)

7-AAD Viability Staining Solution

Go To Top Version: 2 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.