- Clone
- W20041H (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BZS, TEP1, PTEN1, PTENbeta, Phosphatase and tensin homolog, MMAC1, CWS1, DEC, GLM2, MHAM
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W20041H_PURE_PTEN_Antibody_022522 W20041H_PURE_PTEN_Antibody_022522](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W20041H_PURE_PTEN_Antibody_022522.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells (filled histogram, positive control) and PC-3 cells (open histogram, negative control) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-PTEN (clone W20041H) followed by PE goat anti-rat IgG (Cat. No. 405406).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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366201 | 25 µg | $147 |
PTEN was discovered as a tumor suppressor with lipid and protein phosphatase activity. Heterozygous loss of PTEN in mice contribute to the development of a variety of types of cancer, suggesting that insufficient PTEN activity results in increased cancer susceptibility. PTEN negatively regulates the PI3K/AKT pathway by dephosphorylating the second messenger phosphoinositide 3,4,5-triphosphate (PIP3) produced by phosphoinositide 3-kinase (PI3K), which results in inhibition of abnormal cell proliferation. Despite the hydrolysis function of PIP3, the nuclear population of PTEN possesses greater tumor suppressive activities, regulates gene expression, differentiation, and cell cycle progression.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human PTEN protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone failed western blot, IHC, and ICC testing during product development. We therefore do not recommend this product for any of these applications.
- RRID
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AB_2922580 (BioLegend Cat. No. 366201)
Antigen Details
- Structure
- PTEN is a 403 amino acid protein with a predicted molecular weight of 47 kD.
- Distribution
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Ubiquitous/ Nucleus (monoubiquitinated form), Cytoplasm (nonubiquitinated form)
- Function
- Hydrolase, Protein Phosphatase
- Interaction
- Interacts with MAIG2, MAIG3, MAST1, MAST3, NEDD4, NDFIP1, NDFIP2, USP7, ROCK1, and XIAP. The unphosphorylated form interacts with AIP1, DLG1 and MAS.T2.
- Ligand/Receptor
- Lipid-binding
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
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- Carracedo A, et al. 2011. Cancer Res. 71:629-33.
- Jiang BH and Liu LZ. 2009. Adv Cancer Res. 102:19-65.
- Mao JH, et al. 2008. Science 321:1499-502.
- Weng LP, et al. 2001. Hum. Mol. Genet. 10:599-604.
- Wrighton KH. 2011. Nat Rev Cancer. 11:154.
- Wang X, et al. 2007. Cell 128:129-39.
- Gene ID
- 5728 View all products for this Gene ID
- UniProt
- View information about PTEN on UniProt.org
Other Formats
View All PTEN Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PTEN | W20041H | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-PTEN
HeLa cells (filled histogram, positive control) and PC-3 cel...
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