- Other Names
- TetraZ™ Cell Proliferation Kit
- Ave. Rating
- 0 reviews
The TetraZ™ Cell Counting Kit is used to quantify cell proliferation and cell viability. It is based on a water soluble tetrazolium salt that produces a water soluble dye upon reduction in the presence of an electron mediator. Electrons are provided by the action of cellular dehydrogenases which transform the tetrazolium salt into a colored product, formazan. The amount of formazan produced is directly proportional to the number of live cells. Optimal OD is read at 450 nm.Product Details
|Cat #||Size||Price||Quantity Avail.||Save|
- This solution contains WST-8 and Methoxy PMS.
- This solution is ready to use. A 5 ml bottle is sufficient for 500 tests.
- Storage & Handling
- This product is stable for six months at room temperature, and one year when stored between 2°C and 8°C.
FA - Quality tested
- Recommended Usage
Add 10 µl of TetraZ™ Cell Counting solution per 100 µl of cell suspension.
(PubMed link indicates BioLegend citation)
1. Fuda H, et al. 2007. J. Lipid Res. 48:1343.Publication Library
2. Huang DM, et al. 2005. FASEB J. 14:2014.
3. Itano N, et al. 2002. Proc. Natl. Acad. Sci. USA. 6:3609.
4. Ohori H, et al. 2006. Mol. Cancer Ther. 5:2563.
5. Awale S, et al. 2006. Cancer Res. 66:1751.
6. Inoue J, et al. 2004. Am. J. Pathol. 165:71.
7. Furuya F, et al. 2004. Endocrinology 145:2865.
8. Mori T, et al. 2004. Mol. Cancer Ther. 3:29.
9. Awale S, et al. 2006. Cancer Res. 66:1751.
10. Shibata S, et al. 2006. J. Immunol. 177:3564.
- Gene ID
- Can the cells be â€œreusedâ€?
The toxicity of TetraZ is so low, thus, the same cells can be used for downstream applications.
- Can this kit be used with a 24-well (or 384-well) plate?
Yes. Add the TetraZ solution at 1/10 volume of the culture medium in a well. If the volume of TetraZ to be added is too small, dilute TetraZ in half with the medium and add 1/5 of the volume of culture medium in a well.
- Does phenol red affect the assay?
No. The absorption value of phenol red in a culture medium can be removed by subtracting the absorption value of a blank solution from the absorption value of each well.
- Does TetraZ stain viable cells?
No. Since TetraZ and its formazan dye are highly water-soluble, it cannot be utilized for cell staining purpose.
- How many cells should there be in a well?
For adhesive cells, at least 1000 cells are necessary per well (100 μl medium). For leukocytes, at least 2500 cells are necessary per well (100 μl medium). The recommended maximum number of cells per well is 25000 (for a 96-well plate). If a 24-well or 6-well plate is used, please calculate the number of cells per well accordingly, and adjust the volume of the TetraZ solution in a well to 10% of the total volume.
- If I do not have a 450 nm filter, which other filters can I use?
You can use filters between 430 and 490 nm. The 450 nm filter gives the best sensitivity.
- Is there a correlation between TetraZ and the Thymidine incorporation assay?
Yes. However, the results may be different as TetraZ uses a different mechanism from that of the Thymidine assay.