Amyloid precursor protein (APP) is a transmembrane protein that is highly expressed in neuronal tissues and enriched at synapses. APP undergoes proteolytic processing through two distinct pathways: 1) the amyloidogenic pathway that generates amyloid beta (Aβ) peptides through sequential processing by β- and γ-secretases, and 2) the non-amyloidogenic pathway, where processing by α-secretase precludes the generation of Aβ. Aberrant processing and accumulation of Aβ peptides leads to the formation of oligomeric and fibrillar aggregates, which interfere with cellular processes and lead to neuronal cell death. The Aβ aggregates are a hallmark of Alzheimer’s disease (AD) and other Aβ-related disorders, and can be used as a tool for pathology detection.

 

BioLegend’s APP/Aβ Antibody Sampler Kit offers an easy solution for detection of full-length APP and various APP cleavage fragments including Aβ 1-40 and Aβ 1-42 peptides.

APP/Aβ Antibody Sampler Kit contents:

 

Specificity

Clone

Format

Reactivity

Application

β-Amyloid, 1-16

6E10

Purified

Hu

WB, IHC-P, Direct ELISA

β-Amyloid, 1-16

M3.2

Purified

Ms, Rat

WB

β-Amyloid, 1-15

3A1

Purified

Hu

IHC-F, Direct ELISA

β-Amyloid, 1-40

11A50-B10

Purified

Hu

IHC-P

β-Amyloid, 1-42

12F4

Purified

Hu, Ms, Rat

WB, IHC-P, Direct ELISA

Clone 6E10 is a gold standard antibody for human Aβ detection, and it recognizes both Aβ and APP.

 

 

 

 

 

In contrast to 6E10, clone 3A1 demonstrates preference for aggregated forms of Aβ and shows no cross-reactivity with APP.

 

 

 

 

 

Clone 11A50-B10 shows reactivity towards Aβ 1-40, one of the major Aβ species found in the brain.

 

 

 

 

 

Clone 12F4 reacts only with Aβ 1-42 peptide, which readily forms oligomers and can be found at an increased abundance with Alzheimer’s disease.

 

 

 

 

 

Clone M3.2 is rodent-specific and recognizes Aβ, β-CTF, sAPP-α, and full-length APP. Additionally, clone M3.2 has negligible cross-reactivity with human isoforms of APP and Aβ.

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