- Clone
- H57-597 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TCR-β chain, TCR-β, β-TCR
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![h57-597 h57-597](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/h57-597.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC
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T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Affinity purified TCR from mouse DO-11.10 cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
CyTOF® - Verified
IHC-F, IP, Costim, Depletion - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).
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Application References
(PubMed link indicates BioLegend citation) -
- Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
- Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
- Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
- Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
- Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
- van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
- Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
- Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
- Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
- Product Citations
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- RRID
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AB_2813970 (BioLegend Cat. No. 109253)
AB_2813971 (BioLegend Cat. No. 109254)
AB_2813972 (BioLegend Cat. No. 109255)
AB_2813973 (BioLegend Cat. No. 109256)
AB_2813974 (BioLegend Cat. No. 109257)
AB_2813975 (BioLegend Cat. No. 109258)
Antigen Details
- Structure
- Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
- Distribution
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Majority of T cells and thymocytes (correlated to differentiation)
- Function
- Antigen recognition, T cell activation
- Ligand/Receptor
- Peptide bound-MHC class I and II
- Antigen References
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1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736. - Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR beta chain on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 APC -
Biotin anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with biotinylated H57-597,... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
FITC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 FITC -
PE anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ ... -
PE/Cyanine5 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cyanine5. -
Purified anti-mouse TCR β chain
C57L/6 splenocytes stained with H57-597 purified, followed b... Fresh, frozen mouse spleen was stained with purified TCR β c... -
Alexa Fluor® 488 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® ... -
Alexa Fluor® 647 anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Alexa Fluor® 647 C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
APC/Cyanine7 anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
PE/Cyanine7 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cyanine7 -
Alexa Fluor® 700 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor&r... -
Pacific Blue™ anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Pacific Blue™ -
Brilliant Violet 421™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57... -
PerCP/Cyanine5.5 anti-mouse TCR β chain
C57BL/6 splenocytes were stained with CD3e APC and TCR ß cha... -
Brilliant Violet 570™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
Brilliant Violet 510™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57... -
Purified anti-mouse TCR β chain (Maxpar® Ready)
Mouse splenocytes stained with 143Nd-anti-TCRβ (H57-597) and... -
Alexa Fluor® 594 anti-mouse TCR β chain
C57BL/6 mouse frozen lymph node sections were fixed with 4% ... C57BL/6 mouse splenocytes were stained with TCR β (clone H57... -
PE/Dazzle™ 594 anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR-β (clon... -
Brilliant Violet 605™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
Brilliant Violet 711™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
APC/Fire™ 750 anti-mouse TCR β chain
C57BL/6 splenocytes were stained with TCR γ/δ PE... -
TotalSeq™-A0120 anti-mouse TCR β chain
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Brilliant Violet 785™ anti-mouse TCR β chain
C57BL/6 splenocytes were stained with CD3 APC and anti-mouse... -
Brilliant Violet 650™ anti-mouse TCR β chain
C57BL/6 Splenocytes were stained with CD3 APC and anti-mouse... -
Ultra-LEAF™ Purified anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained with H57-597 PE ... -
TotalSeq™-C0120 anti-mouse TCR β chain
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TotalSeq™-B0120 anti-mouse TCR β chain
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Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™)
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Spark PLUS UV™ 395 anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ...
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