- Clone
- 3F10-B3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-8RB, GRO/MGSA, Cmkar2
- Isotype
- Mouse IgG2c, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_3F10B3_ULEAF_CD182_Antibody_060622 A_3F10B3_ULEAF_CD182_Antibody_060622](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3F10B3_ULEAF_CD182_Antibody_060622.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with anti-mouse Ly-6G/Ly-6C APC and Ultra-LEAF™ Purified anti-mouse CXCR2 (clone 3F10-B3) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a. -
Recombinant mouse CXCL2 (5 ng/mL) (Cat. No 582502) chemoattracts Baf3-mCXCR2 transfected cells (black circles) in a dose-dependent manner. Anti-mouse CXCR2 (clone 3F10-B3) inhibits the migration of Baf3-mCXCR2 transfected cells (purple circles), in a dose dependent manner. The ED50 for this effect is 1 - 4 µg/mL.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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163903 | 100 µg | $292 | ||||
163904 | 1 mg | $743 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Pre-B cell transfected with mCXCR2
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone 3F10-B3 blocks the chemotaxis towards CXCR2 ligands.
- RRID
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AB_2927843 (BioLegend Cat. No. 163903)
AB_2927843 (BioLegend Cat. No. 163904)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions.
- Distribution
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Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK)
- Function
- Chemotaxis, neutrophil activation, and angiogenesis
- Ligand/Receptor
- CXCL1, CXCL2, CXCL3, and CXCL5
- Cell Type
- Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
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- Spaan AN, et al. 2014. Nat Commun. 5:5438.
- Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064-9.
- Hsieh CY, et al. 2014. J Immunol. 193:3693-703.
- Gene ID
- 12765 View all products for this Gene ID
- UniProt
- View information about CD182 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD182 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2) | 3F10-B3 | FC,Block |
Purified anti-mouse CD182 (CXCR2) | 3F10-B3 | FC,Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... Recombinant mouse CXCL2 (5 ng/mL) (Cat. No 582502) chemoattr... -
Purified anti-mouse CD182 (CXCR2)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... Recombinant mouse CXCL2 (MIP-2) (carrier-free) (5 ng/mL) (Ca...
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