- Clone
- W21007F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BST1
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W21007F_ULEAF_CD157_Antibody_1_122022 W21007F_ULEAF_CD157_Antibody_1_122022](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W21007F_ULEAF_CD157_Antibody_1_122022.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with anti-human CD14 APC/Cyanine7 and purified anti-human CD157 (clone W21007F) (left) or purified rat IgG2a, λ isotype control (right) followed by anti-rat IgG APC and anti-human CD45 Pacific Blue™. Data shown was gated on CD45-positive lymphocytes+ monocytes. -
The myelomonocytic cell line U937 was labeled with Calcein AM (Cat. No. 425201), washed and plated in 96 well plates with the indicated concentration of Ultra-LEAF™ purified anti-human CD157 (clone W21007F) or rat IgG2a, λ isotype control. After 45 minutes, Calcein fluorescence was measured using a SpectraMax plate reader. -
Human peripheral blood mononuclear cells were treated with the indicated concentration of Ultra-LEAF™ purified anti-human CD157 (clone W21007F) or rat IgG2a, λ isotype control and plated on tissue culture plates coated with 50 µg/mL recombinant human FN-1 (carrier-free) (Cat. No. 775306). After 1 hour, non-adherent cells were removed by washing and adherent cells counted by brightfield microscopy. Data shown are fold changes normalized to isotype controls. -
The myelomonocytic cell line U937 was plated onto chamber slides coated with recombinant human FN-1 with 5 µg/mL of the indicated antibodies and incubated for 1 hour at 37°C. Media was removed and cells were fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Cells were washed twice with 1X Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) and then stained with 5 µg/mL of Ultra-LEAF™ purified anti-human CD157 (clone W21007F) (red), 5 µg/mL of Alexa Fluor® 647 anti-human CD29 (clone TS2/16) (blue) and Flash Phalloidin™ Green 488 (Cat. No. 424201) (green). Cells were washed 3 times with 1X Intracellular Staining Permeabilization Wash Buffer and then stained with Alexa Fluor® 555 anti-rat IgG (Cat. No. 405420) for 1 hour and imaged using a 40x objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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382105 | 100 µg | $325 | ||||
382106 | 1 mg | $1205 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST1), is a GPI-anchored glycoprotein. It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. It is prevalently expressed by cells of the myelomonocytic lineage, and modulates adhesion of neutrophils and monocytes to the extracellular matrix. CD157 is expressed in 97% of primary acute myeloid leukemia samples, and is used as a marker for diagnosis of Paroxysmal Nocturnal Hemoglobinuria, a disease caused by intrinsic deficiency of GPI anchors.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- hCD157 stable transfectant followed by recombinant protein (aa29-293)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
Block, Activ, ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For blocking of monocyte adhension, a concentration range of 1.25 – 5.0 µg/mL is suggested. For activation of signaling, a concentration range of 2.5 – 10.0 μg/mL is recommended. For immunocytochemistry, the suggested use of this reagent is 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
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This clone blocks adhesion of monocytes to fibronectin.
This clone activates calcium signaling in monocytes and induces cell polarization and actin polymerization. - RRID
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AB_2936535 (BioLegend Cat. No. 382105)
Antigen Details
- Structure
- GPI-anchored, ADP-ribosyl cyclase
- Distribution
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Cell Membrane
- Function
- ADP-ribosyl cyclase activity. Fibronectin, fibrinogen, laminin-1 and type 1 collagen binding. Hydrolase, Transferase.
- Interaction
- CD11b/CD18, ECM components
- Ligand/Receptor
- NAD, NADP
- Cell Type
- Antigen-presenting cells, Granulocytes, Monocytes
- Biology Area
- Cell Adhesion, Cell Proliferation and Viability, Innate Immunity
- Molecular Family
- CD Molecules, Enzymes and Regulators, Innate Immune Signaling
- Antigen References
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- Ortolan E, et al. 2019. Immunology Letters. 205:59-64
- Ferrero E, et al. 2017. Sci Rep. 7: 15923.
- Kaisho T, et al. 1994. Proc Natl Acad Sci USA. 91:5325-9.
- Morone S, et al. 2014. J Biol Chem. 289:15588-601.
- Gene ID
- 683 View all products for this Gene ID
- UniProt
- View information about CD157 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD157 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD157 (BST-1) | W21007F | FC |
PE anti-human CD157 (BST-1) | W21007F | FC |
Ultra-LEAF™ Purified anti-human CD157 | W21007F | FC,Activ,ICC,Block |
APC anti-human CD157 (BST-1) | W21007F | FC |
PE/Dazzle™ 594 anti-human CD157 (BST-1) | W21007F | FC |
APC/Fire™ 750 anti-human CD157 (BST-1) | W21007F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD157 (BST-1)
Human peripheral blood mononuclear cells were stained with a... -
PE anti-human CD157 (BST-1)
Human peripheral blood mononuclear cells were stained with a... -
Ultra-LEAF™ Purified anti-human CD157
Human peripheral blood mononuclear cells were stained with a... The myelomonocytic cell line U937 was labeled with Calcein A... Human peripheral blood mononuclear cells were treated with t... The myelomonocytic cell line U937 was plated onto chamber sl... -
APC anti-human CD157 (BST-1)
Human peripheral blood mononuclear cells were stained with a... -
PE/Dazzle™ 594 anti-human CD157 (BST-1)
Human peripheral blood mononuclear cells were stained with a... -
APC/Fire™ 750 anti-human CD157 (BST-1)
Human peripheral blood mononuclear cells were stained with a...
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