- Clone
- SHG-1 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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The SHG-1 immunoglobulin is of Syrian hamster IgG isotype and was chosen as an isotype control after screening on variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- Trinitrophenol + KLH
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment. - Application Notes
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The SHG-1 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 402014) as negative control.
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Application References
(PubMed link indicates BioLegend citation) -
- Nishimoto H, et al. 2005. Blood. 106:4241.
- Product Citations
-
- RRID
-
AB_3097020 (BioLegend Cat. No. 402019)
AB_3097020 (BioLegend Cat. No. 402020)
AB_3097020 (BioLegend Cat. No. 402021)
AB_3097020 (BioLegend Cat. No. 402022)
AB_3097020 (BioLegend Cat. No. 402023)
AB_3097020 (BioLegend Cat. No. 402024)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Other Formats
View All ReagentsDescription | Clone | Applications |
---|---|---|
Purified Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC,WB,IP,ICC,IHC |
Biotin Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
FITC Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
PE Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
PE/Cyanine5 Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
APC Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
Alexa Fluor® 488 Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
PE/Cyanine7 Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
GoInVivo™ Purified Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC,WB,IP,ICC,IHC,FA |
PerCP/Cyanine5.5 Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
PE/Dazzle™ 594 Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC |
Ultra-LEAF™ Purified Syrian Hamster IgG Isotype Ctrl | SHG-1 | FC,ICFC,WB,IP,ICC,IHC,FA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified Syrian Hamster IgG Isotype Ctrl
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Biotin Syrian Hamster IgG Isotype Ctrl
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FITC Syrian Hamster IgG Isotype Ctrl
-
PE Syrian Hamster IgG Isotype Ctrl
-
PE/Cyanine5 Syrian Hamster IgG Isotype Ctrl
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APC Syrian Hamster IgG Isotype Ctrl
-
Alexa Fluor® 488 Syrian Hamster IgG Isotype Ctrl
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PE/Cyanine7 Syrian Hamster IgG Isotype Ctrl
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GoInVivo™ Purified Syrian Hamster IgG Isotype Ctrl
-
PerCP/Cyanine5.5 Syrian Hamster IgG Isotype Ctrl
-
PE/Dazzle™ 594 Syrian Hamster IgG Isotype Ctrl
-
Ultra-LEAF™ Purified Syrian Hamster IgG Isotype Ctrl
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