Webinar: Revolutionizing Cell Health Monitoring with Apotracker

Annexin V staining is a widely established method to detect apoptotic and necrotic cells. However, the Annexin V staining procedure exposes cells to specialized binding buffers that contain a high concentration of calcium ions, which itself induces undesired cytotoxic effects. In this webinar, Dr. Kenta Yamamoto introduces our novel Apotracker™ reagents, which bind to phosphatidylserine exposed on apoptotic and necrotic cells through calcium-independent mechanisms. Apotracker™ binding can be performed in standard flow cytometry staining buffers, reducing cytotoxicity risk from the use of specialized buffers. In addition to probing for cell death, Apotracker™ has paved the way for additional innovative reagents and applications, including our dead cell removal kits, and extracellular vesicle identification on leukocytes post-immune response in vitro and in vivo. Thus, Apotracker™ provides a variety of advantages over existing Annexin V staining-based methods and is poised to become a new standard in measuring cell health.

Key applications to the cytometry community:
Detection of apoptotic and necrotic cells in flow cytometry, through calcium-independent mechanisms.