Webinar: Using a Cell Surface Screening Tool to Identify Novel Markers of Lymphoid Primed Progenitors

New research is challenging the classical view of hematopoiesis—in which the earliest commitment toward the lymphoid lineage is described within a multipotent progenitor population termed lymphoid-primed multipotent progenitors (LMPPs). These cells are Lin^–cKit^hiSca1⁺Flt3⁺ and devoid of erythroid and megakaryocytic potential but still produce myeloid cells.

In this webinar, Sara Tomei of Shalin Naik’s group at The Walter & Eliza Hall Institute, will discuss the group’s recently identified subpopulation of LMPPs marked by Dach1-GFP downregulation. This subpopulation, termed lymphoid primed progenitors (LPPs), is completely devoid of any myeloid potential but can produce B and T cells. She will also explain how the team used LEGENDScreen™ to identify potential surface markers that could act as surrogates for LPPs.

Through staining for 263 markers, the group identified CD69 as a novel candidate marker expressed on this population. This crucial finding allows any researcher to prospectively isolate this population without the need for Dach1-GFP reporter mice.

Topics to be covered

• The significance of lymphoid primed progenitors (LPPs) in hematopoietic stem and progenitor populations
• How to isolate lymphoid primed progenitors (LPPs) without a Dach1-GFP reporter mouse
• Lessons learned running LEGENDScreen™, and how other novel markers of heterogeneity can be revealed through this data set