Webinar: Evaluation of Large Antibody Panels in Single-Cell Genomic Immunophenotyping of Fresh and Frozen Human Leukocytes

Multiomic single-cell studies are revolutionizing knowledge of the tumor immune microenvironment and are uniquely positioned to detect rare cell subsets that correlate in response to immunotherapy. DNA-barcoded antibody panels enable unambiguous classification of cell subsets via simultaneous measurement of surface proteins and the transcriptome. However, single-cell sequencing performs best on fresh samples while multicenter clinical trials routinely obtain frozen specimens.

 

Dr. Jasmine Chen shares Abiosciences’ effort in evaluating BioLegend’s TotalSeq™-C Human Universal Cocktail using the 10x Genomics single-cell platform on fresh and frozen PBMCs. This includes bioinformatics pipelines to compare the quality control metrics, isotype matched control performance and cell subtype analyses. Advanced visualization and analysis tools were developed to interrogate the data in a flow cytometry paradigm, including complex gating strategies to identify rare cell subtypes. The pilot study is being expanded to larger-scale clinical settings and may help characterize important cell populations that are associated with disease status, pharmacodynamics, and therapeutic response.

 

In this webinar you will learn:

• Multimodal single-cell genomics show that fresh and frozen PBMC samples are largely concordant for transcriptome and cell-surface protein levels
• How bioinformatics approaches allow rare cell subtype ID with sensitivity and selectivity rivaling flow cytometry

Learn more about our TotalSeq reagents