- Clone
- HM48-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BCM1, SLAMF2, Blast-1
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified HM48-1, followed by anti-Armennian hamster IgG FITC
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CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T lymphoma MBL-2
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
- Application References
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- Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
- Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
- RRID
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AB_2832312 (BioLegend Cat. No. 103456)
AB_2832310 (BioLegend Cat. No. 103454)
AB_2832311 (BioLegend Cat. No. 103455)
AB_2832308 (BioLegend Cat. No. 103452)
AB_2832309 (BioLegend Cat. No. 103453)
Antigen Details
- Structure
- Ig superfamily, 45 kD
- Distribution
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T cells and B cells, monocytes/macrophages
- Function
- Adhesion, T cell recognition
- Ligand/Receptor
- CD2, CD244
- Cell Type
- B cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809. - Gene ID
- 12506 View all products for this Gene ID
- UniProt
- View information about CD48 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD48 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 FITC -
PE anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 PE -
Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with purified HM48-1, foll... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Biotin anti-mouse CD48
C57BL/6 mouse splenocytes stained with biotinylated HM48-1, ... -
APC anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 APC -
Alexa Fluor® 488 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor&re... -
Alexa Fluor® 647 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor&re... -
Pacific Blue™ anti-mouse CD48
C57BL/6 mouse splenocytes stained with Pacific blue™ H... -
PE/Cyanine5 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cyanine5 -
PerCP/Cyanine5.5 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
PE/Cyanine7 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cyanine7 -
Alexa Fluor® 700 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700 -
Brilliant Violet 421™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
APC/Cyanine7 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Purified anti-mouse CD48 (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
Brilliant Violet 605™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Brilliant Violet 510™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Brilliant Violet 711™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
PE/Dazzle™ 594 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
APC/Fire™ 750 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
TotalSeq™-A0429 anti-mouse CD48
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Brilliant Violet 785™ anti-mouse CD48
C57BL/6 splenocytes were stained with CD48 (clone HM48-1) Br... -
Ultra-LEAF™ Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
TotalSeq™-B0429 anti-mouse CD48
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TotalSeq™-C0429 anti-mouse CD48
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