- Clone
- DX2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI C-64
- Other Names
- Fas, APO-1, TNFRSF6
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![DX2_LEAF_070507 DX2_LEAF_070507](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/DX2_LEAF_070507.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified DX2, followed by anti-mouse IgGs FITC -
MCF7 breast cancer cells were stained with anti-human CD95 (clone DX2) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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305655 | 100 µg | 216€ | ||||
305656 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD95 transfected L cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
ICC - Verified
IHC, Apop - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).
Note: EOS9.1 antibody can induce apoptosis without cross-linking. - Application References
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- Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
- Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
- Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
- Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
- Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
- RRID
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AB_2860812 (BioLegend Cat. No. 305655)
AB_2860813 (BioLegend Cat. No. 305656)
Antigen Details
- Structure
- TNFR superfamily, type I transmembrane protein, 45 kD
- Distribution
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T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
- Function
- Mediates apoptosis
- Ligand/Receptor
- Fas ligand (CD178)
- Cell Type
- B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
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1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449. - Gene ID
- 355 View all products for this Gene ID
- UniProt
- View information about CD95 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD95 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 APC -
Biotin anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 FITC -
PE anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 PE -
PE/Cyanine5 anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 PE/Cyani... -
Purified anti-human CD95 (Fas)
Human peripheral blood lymphocytes stainedwith DX2 PE/Cyanin... MCF7 breast cancer cells were stained with anti-human CD95 (... -
Alexa Fluor® 488 anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 Alexa Fl... -
Alexa Fluor® 647 anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 Alexa Fl... -
Brilliant Violet 421™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
Pacific Blue™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 Pacific ... -
PE/Cyanine7 anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 PE/Cyani... -
Brilliant Violet 605™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
PerCP/Cyanine5.5 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
Purified anti-human CD95 (Fas) (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 164Dγ-an... -
PE/Dazzle™ 594 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
APC/Fire™ 750 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
APC/Cyanine7 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (F... -
Brilliant Violet 510™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
Brilliant Violet 711™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
Brilliant Violet 785™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
Brilliant Violet 650™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (c... -
Alexa Fluor® 700 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with CD95 (F... -
TotalSeq™-A0156 anti-human CD95 (Fas)
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TotalSeq™-C0156 anti-human CD95 (Fas)
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TotalSeq™-B0156 anti-human CD95 (Fas)
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Ultra-LEAF™ Purified anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ... MCF7 breast cancer cells were stained with anti-human CD95 (... -
TotalSeq™-D0156 anti-human CD95 (Fas)
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PE/Fire™ 640 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with anti-hu... -
KIRAVIA Blue 520™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD95 (Fas) Antibody
Human peripheral lymphocytes were stained with anti-human CD... -
PE anti-human CD95
Typical results from human peripheral blood lymphocytes stai... -
Spark YG™ 593 anti-human CD95 (Fas)
Human peripheral blood monocytes were stained with anti-huma... Human peripheral blood monocytes were stained with anti-huma... -
PE/Fire™ 700 anti-human CD95 (Fas)
Human peripheral blood monocytes were stained with anti-huma... Human peripheral blood monocytes were stained with anti-huma... -
PerCP/Fire™ 780 anti-human CD95 (Fas)
Human peripheral blood monocytes were stained with anti-huma... Human peripheral blood monocytes were stained with anti-huma... -
Spark Blue™ 574 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD95 (Fas)
Human peripheral blood lymphocytes were stained with anti-hu...
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