- Clone
- 5C3 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD40.4
- Other Names
- BP50, TNFRSF5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes stained with LEAF™ purified 5C3, followed by anti-mouse IgG FITC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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334350 | 100 µg | 190€ | ||||
334351 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
Costim - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
- Application References
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- Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pound JD, et al. 1999. Int. Immunol. 11:11.
- Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
- Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
- Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
- Product Citations
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- RRID
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AB_2810512 (BioLegend Cat. No. 334350)
AB_2810513 (BioLegend Cat. No. 334351)
Antigen Details
- Structure
- TNFR superfamily, type I glycoprotein, 48 kD
- Distribution
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B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
- Function
- B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
- Ligand/Receptor
- CD154 (CD40 ligand)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591. - Gene ID
- 958 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD40 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 488 anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 Alexa Fl... -
Purified anti-human CD40
Human peripheral blood lymphocytes stained with purified 5C3... -
FITC anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 FITC -
PE anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 PE -
APC anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 APC -
Alexa Fluor® 647 anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 Alexa Fl... -
PerCP/Cyanine5.5 anti-human CD40
Human peripheral blood lymphocytes stained with CD19 APC and... -
Pacific Blue™ anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 Pacific ... -
PE/Cyanine7 anti-human CD40
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Cyanine7 anti-human CD40
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 700 anti-human CD40
Human peripheral blood lymphocytes stained with CD40 (clone ... -
Purified anti-human CD40 (Maxpar® Ready)
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 165Ho-a... -
Brilliant Violet 510™ anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
Brilliant Violet 421™ anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
Brilliant Violet 711™ anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
Brilliant Violet 605™ anti-human CD40
Human peripheral blood lymphocytes stained with CD40 (clone ... -
Brilliant Violet 650™ anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
Brilliant Violet 785™ anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
PE/Dazzle™ 594 anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
Biotin anti-human CD40
Human peripheral blood lymphocytes were stained with biotiny... -
APC/Fire™ 750 anti-human CD40
Human peripheral blood lymphocytes were stained with CD40 (c... -
TotalSeq™-A0031 anti-human CD40
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TotalSeq™-C0031 anti-human CD40
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Ultra-LEAF™ Purified anti-human CD40
Human peripheral blood lymphocytes stained with LEAF™ purifi... -
TotalSeq™-B0031 anti-human CD40
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TotalSeq™-D0031 anti-human CD40
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PE/Fire™ 810 anti-human CD40
Human peripheral blood lymphocytes stained with anti-human C...
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