- Clone
- RTK2071 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
ICFC, ICC, IHC, WB, IP, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this rat IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
- Application Notes
-
The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
- Application References
- Product Citations
-
- RRID
-
AB_11150233 (BioLegend Cat. No. 400462)
AB_11150233 (BioLegend Cat. No. 400458)
AB_11150233 (BioLegend Cat. No. 400461)
AB_11150233 (BioLegend Cat. No. 400431)
AB_11150772 (BioLegend Cat. No. 400432)
AB_11150233 (BioLegend Cat. No. 400457)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Rat IgG1, κ Isotype Ctrl
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Biotin Rat IgG1, κ Isotype Ctrl
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FITC Rat IgG1, κ Isotype Ctrl
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PE Rat IgG1, κ Isotype Ctrl
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PE/Cyanine5 Rat IgG1, κ Isotype Ctrl
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Purified Rat IgG1, κ Isotype Ctrl
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PE/Cyanine7 Rat IgG1, κ Isotype Ctrl
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Alexa Fluor® 647 Rat IgG1, κ Isotype Ctrl
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Alexa Fluor® 488 Rat IgG1, κ Isotype Ctrl
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Pacific Blue™ Rat IgG1, κ Isotype Ctrl
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Alexa Fluor® 700 Rat IgG1, κ Isotype Ctrl
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APC/Cyanine7 Rat IgG1, κ Isotype Ctrl
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PerCP Rat IgG1, κ Isotype Ctrl
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PerCP/Cyanine5.5 Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 421™ Rat IgG1, κ Isotype Ctrl
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Ultra-LEAF™ Purified Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 605™ Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 510™ Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 650™ Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 711™ Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 785™ Rat IgG1, κ Isotype Ctrl
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PE/Dazzle™ 594 Rat IgG1, κ Isotype Ctrl
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Alexa Fluor® 594 Rat IgG1, κ Isotype Ctrl
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GoInVivo™ Purified Rat IgG1, κ Isotype Ctrl
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APC/Fire™ 750 Rat IgG1, κ Isotype Ctrl
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TotalSeq™-A0236 Rat IgG1, κ Isotype Ctrl
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KIRAVIA Blue 520™ Rat IgG1, κ Isotype Ctrl
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TotalSeq™-B0236 Rat IgG1, κ Isotype Ctrl
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TotalSeq™-C0236 Rat IgG1, κ Isotype Ctrl
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Spark YG™ 581 Rat IgG1, κ Isotype Ctrl
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Spark YG™ 593 Rat IgG1, κ Isotype Ctrl
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Spark NIR™ 685 Rat IgG1, κ Isotype Ctrl
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PE/Fire™ 640 Rat IgG1, κ Isotype Ctrl
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TotalSeq™-D0236 Rat IgG1, κ Isotype Ctrl
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PE/Fire™ 700 Rat IgG1, κ Isotype Ctrl
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APC/Fire™ 810 Rat IgG1, κ Isotype Ctrl
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Brilliant Violet 750™ Rat IgG1, κ Isotype Ctrl
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Spark YG™ 570 Rat IgG1, κ Isotype Ctrl
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PerCP/Fire™ 806 Rat IgG1, κ Isotype Ctrl
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PerCP/Fire™ 780 Rat IgG1, κ Isotype Ctrl
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Spark Red™ 718 Rat IgG1, κ Isotype Ctrl
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TotalSeq™-Bn0236 Rat IgG1, κ Isotype Ctrl
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PE/Fire™ 744 Rat IgG1, κ Isotype Ctrl
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