- Clone
- RTK2758 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this rat IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
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Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
- Application References
-
- Nishimoto H, et al. 2005. Blood 106:4241.
- Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
- Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
- Product Citations
-
- RRID
-
AB_11147167 (BioLegend Cat. No. 400574)
AB_11147167 (BioLegend Cat. No. 400566)
AB_11147167 (BioLegend Cat. No. 400573)
AB_11148951 (BioLegend Cat. No. 400543)
AB_11147167 (BioLegend Cat. No. 400544)
AB_11147167 (BioLegend Cat. No. 400565)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Rat IgG2a, κ Isotype Ctrl
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Biotin Rat IgG2a, κ Isotype Ctrl
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FITC Rat IgG2a, κ Isotype Ctrl
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PE Rat IgG2a, κ Isotype Ctrl
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PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl
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Purified Rat IgG2a, κ Isotype Ctrl
Chromatin Immunoprecipitations (ChIP) were performed with cr... -
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl
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APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl
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Pacific Blue™ Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl
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PerCP Rat IgG2a, κ Isotype Ctrl
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PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl
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Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl
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PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl
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GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl
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APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl
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KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl
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Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl
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APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl
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Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl
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Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl
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Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl
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Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl
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Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl
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Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl
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Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl
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PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl
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Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl
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PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl
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Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl
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Spark UV™ 387 Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 744 Rat IgG2a, κ Isotype Ctrl
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Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl
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