- Clone
- RTK4530 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this rat IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
-
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
- Additional Product Notes
-
View more applications data for this product in our Scientific Poster Library.
Watch a Scientific Poster video of this product. - Application References
-
- Cervantes-Barragan L, et al. 2007. Blood 109:1131.
- Zeiser R, et al. 2007. Blood 109:2225.
- Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
- Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
- Yi H, et al. 2009. Blood 113:5819. PubMed
- Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
- Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
- Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
- Product Citations
-
- RRID
-
AB_11147763 (BioLegend Cat. No. 400672)
AB_11147763 (BioLegend Cat. No. 400675)
AB_11147763 (BioLegend Cat. No. 400676)
AB_11147763 (BioLegend Cat. No. 400643)
AB_11149687 (BioLegend Cat. No. 400644)
AB_11147763 (BioLegend Cat. No. 400671)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Rat IgG2b, κ Isotype Ctrl
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Biotin Rat IgG2b, κ Isotype Ctrl
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FITC Rat IgG2b, κ Isotype Ctrl
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PE Rat IgG2b, κ Isotype Ctrl
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PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl
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Purified Rat IgG2b, κ Isotype Ctrl
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PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl
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APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl
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Pacific Blue™ Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl
CD57BL/6 mouse splenocytes stained with RTK4530 Alexa Fluor®... -
PerCP Rat IgG2b, κ Isotype Ctrl
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PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl
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Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl
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PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl
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GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl
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APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl
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KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl
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APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl
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Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl
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Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl
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PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl
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PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl
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Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-Bn0095 Rat IgG2b, κ Isotype Ctrl
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Spark Violet™ 538 Rat IgG2b, κ Isotype Ctrl
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Spark UV™ 387 Rat IgG2b, κ Isotype Ctrl
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Spark PLUS UV™ 395 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 744 Rat IgG2b, κ Isotype Ctrl
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