- Clone
- RTK4530 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment. - Application Notes
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GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. - Additional Product Notes
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View more applications data for this product in our Scientific Poster Library.
- Application References
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- Cervantes-Barragan L, et al. 2007. Blood 109:1131.
- Zeiser R, et al. 2007. Blood 109:2225.
- Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
- Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
- Yi H, et al. 2009. Blood 113:5819. PubMed
- Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
- Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
- Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
- Product Citations
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- RRID
-
AB_3097011 (BioLegend Cat. No. 400663)
AB_3097011 (BioLegend Cat. No. 400665)
AB_3097011 (BioLegend Cat. No. 400664)
AB_3097011 (BioLegend Cat. No. 400667)
AB_3097011 (BioLegend Cat. No. 400668)
AB_3097011 (BioLegend Cat. No. 400666)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
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Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Rat IgG2b, κ Isotype Ctrl
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Biotin Rat IgG2b, κ Isotype Ctrl
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FITC Rat IgG2b, κ Isotype Ctrl
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PE Rat IgG2b, κ Isotype Ctrl
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PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl
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Purified Rat IgG2b, κ Isotype Ctrl
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PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl
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APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl
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Pacific Blue™ Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl
CD57BL/6 mouse splenocytes stained with RTK4530 Alexa Fluor®... -
PerCP Rat IgG2b, κ Isotype Ctrl
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PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl
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Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl
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PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl
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GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl
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APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl
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KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl
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APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl
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Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl
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Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl
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PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl
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PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl
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Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-Bn0095 Rat IgG2b, κ Isotype Ctrl
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Spark Violet™ 538 Rat IgG2b, κ Isotype Ctrl
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Spark UV™ 387 Rat IgG2b, κ Isotype Ctrl
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Spark PLUS UV™ 395 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 744 Rat IgG2b, κ Isotype Ctrl
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