- Clone
- R1-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α4 integrin, VLA-4 α chain, integrin α4, ITGA4
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![R1-2_LEAF_090507.jpg R1-2_LEAF_090507.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/R1-2_LEAF_090507.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified R1-2, followed by anti-rat IgG FITC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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103629 | 100 µg | 187 CHF | ||||
103630 | 1 mg | 677 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- AKR/Cum mouse spontaneous T lymphoma line TK1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IP, Block, IHC-F - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation1-4, immunoprecipitation1, and immunohistochemistry of frozen sections5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103629 and 103630).
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Application References
(PubMed link indicates BioLegend citation) -
- Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
- Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
- Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
- Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
- Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- RRID
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AB_2832313 (BioLegend Cat. No. 103629)
AB_2832314 (BioLegend Cat. No. 103630)
Antigen Details
- Structure
- Integrin family, associates with integrin β1 or β7, 150 kD
- Distribution
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T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
- Function
- T cell costimulation, cell-cell and cell-matrix adhesion
- Ligand/Receptor
- VCAM-1, MAdCAM-1, fibronectin
- Cell Type
- B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079. - Gene ID
- 16401 View all products for this Gene ID
- UniProt
- View information about CD49d on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD49d Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD49d | R1-2 | FC |
FITC anti-mouse CD49d | R1-2 | FC |
PE anti-mouse CD49d | R1-2 | FC |
Alexa Fluor® 488 anti-mouse CD49d | R1-2 | FC |
Alexa Fluor® 647 anti-mouse CD49d | R1-2 | FC |
PE/Cyanine7 anti-mouse CD49d | R1-2 | FC |
PerCP/Cyanine5.5 anti-mouse CD49d | R1-2 | FC |
APC anti-mouse CD49d | R1-2 | FC |
TotalSeq™-A0078 anti-mouse CD49d | R1-2 | PG |
APC/Fire™ 750 anti-mouse CD49d | R1-2 | FC |
PE/Dazzle™ 594 anti-mouse CD49d | R1-2 | FC |
Ultra-LEAF™ Purified anti-mouse CD49d | R1-2 | FC,IP,Block,IHC-F |
TotalSeq™-B0078 anti-mouse CD49d | R1-2 | PG |
TotalSeq™-C0078 anti-mouse CD49d | R1-2 | PG |
APC/Cyanine7 anti-mouse CD49d | R1-2 | FC |
Brilliant Violet 785™ anti-mouse CD49d | R1-2 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD49d
C57BL/6 mouse splenocytes stained with biotinylated R1-2, fo... -
FITC anti-mouse CD49d
C57BL/6 splenocytes stained with R1-2 FITC -
PE anti-mouse CD49d
C57BL/6 splenocytes stained with R1-2 PE -
Alexa Fluor® 488 anti-mouse CD49d
C57BL/6 mouse splenocytes stained with R1-2 Alexa Fluor® 488 -
Alexa Fluor® 647 anti-mouse CD49d
C57BL/6 mouse splenocytes stained with R1-2 Alexa Fluor® 647 -
PE/Cyanine7 anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with CD49d (clone R1-... -
PerCP/Cyanine5.5 anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with CD49d (clone R1-... -
APC anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with CD49d (clone R1-... -
TotalSeq™-A0078 anti-mouse CD49d
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APC/Fire™ 750 anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with CD49d (clone R1-... -
PE/Dazzle™ 594 anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with CD49d (clone R1-... -
Ultra-LEAF™ Purified anti-mouse CD49d
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
TotalSeq™-B0078 anti-mouse CD49d
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TotalSeq™-C0078 anti-mouse CD49d
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APC/Cyanine7 anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... -
Brilliant Violet 785™ anti-mouse CD49d
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (...
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