- Clone
- 1B1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD1, CD1.1, Ly-38
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with Ultra-LEAF™ Purified 1B1, followed anti-rat IgG FITC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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123531 | 100 µg | 165 CHF | ||||
123532 | 1 mg | 677 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has been shown that PKCδ is a critical regulator of CD1d-mediated antigen presentation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IHC, IP, Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3.
This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.
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Application References
(PubMed link indicates BioLegend citation) -
- Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
- Brozovic S, et al. 2004. Nat. Med. 10:535.
- Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
- Jiang J, et al. 2012. PLoS One. 7:47487. PubMed
- RRID
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AB_2832442 (BioLegend Cat. No. 123531)
AB_2832443 (BioLegend Cat. No. 123532)
Antigen Details
- Structure
- 48 kD glycoprotein, Ig superfamily
- Distribution
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leukocytes, dendritic cells, epithelial cells, thymocytes
- Function
- Antigen presentation
- Ligand/Receptor
- similar to MHC class I, associate with ß-microglobulin
- Cell Type
- Dendritic cells, Epithelial cells, Leukocytes, Thymocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Brudin N, et al. 1998. J. Immunol. 161:3271.
2. Amano M, et al. 1998. J. Immunol. 161:1710.
3. Brossay L, et al. 1997. J. Immunol. 159:1216.
4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113.
5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390. - Gene ID
- 12479 View all products for this Gene ID
- UniProt
- View information about CD1d on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD1d Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC,IHC,IP |
Biotin anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC,IHC |
FITC anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
PE anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
Alexa Fluor® 647 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
PerCP/Cyanine5.5 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
Pacific Blue™ anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
PE/Dazzle™ 594 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
APC anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
PE/Cyanine7 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
APC/Fire™ 750 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
Brilliant Violet 421™ anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC |
TotalSeq™-A0851 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | PG |
Ultra-LEAF™ Purified anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | FC,Block,IHC,IP |
TotalSeq™-C0851 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | PG |
TotalSeq™-B0851 anti-mouse CD1d (CD1.1, Ly-38) | 1B1 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes stained with purified 1B1, followe... -
Biotin anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes stained with biotinylated 1B1, fol... -
FITC anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 splenocytes stained with 1B1 FITC -
PE anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 splenocytes stained with 1B1 PE -
Alexa Fluor® 647 anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 splenocytes stained with 1B1 Alexa Fluor® 647 -
PerCP/Cyanine5.5 anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes stained with 1B1 PerCP/Cyanine5.5 -
Pacific Blue™ anti-mouse CD1d (CD1.1, Ly-38)
C57/B6 mouse splenocytes were stained with CD1d (clone 1B1) ... -
PE/Dazzle™ 594 anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes were stained with CD1d PE/Dazzle™ ... -
APC anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes were stained with CD1d APC (clone ... -
PE/Cyanine7 anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes were stained with CD1d PE/Cyanine7... -
APC/Fire™ 750 anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes were stained with B220 FITC and CD... -
Brilliant Violet 421™ anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
TotalSeq™-A0851 anti-mouse CD1d (CD1.1, Ly-38)
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Ultra-LEAF™ Purified anti-mouse CD1d (CD1.1, Ly-38)
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ Purified ... -
TotalSeq™-C0851 anti-mouse CD1d (CD1.1, Ly-38)
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TotalSeq™-B0851 anti-mouse CD1d (CD1.1, Ly-38)
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