- Clone
- P84 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P84_Pure_1_051012 P84_Pure_1_051012](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P84_Pure_1_051012.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with CD11b APC and Ultra-LEAF™ purified CD172a (clone P84, top) or purified rat IgG1, κ isotype control (bottom), followed by anti-rat IgG1 FITC. Data shown was gated on the myeloid cell population. -
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CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse brain membrane protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IHC-F, IP, Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.
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Application References
(PubMed link indicates BioLegend citation) -
- Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
- Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
- Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
- Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- RRID
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AB_2832521 (BioLegend Cat. No. 144040)
AB_2832519 (BioLegend Cat. No. 144038)
AB_2832520 (BioLegend Cat. No. 144039)
AB_2832516 (BioLegend Cat. No. 144035)
AB_2832517 (BioLegend Cat. No. 144036)
AB_2832518 (BioLegend Cat. No. 144037)
Antigen Details
- Structure
- Type I transmembrane protein
- Distribution
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Monocytes, macrophages, myeloid cells, neuronal tissue
- Function
- Negative regulation of several biological processes
- Interaction
- PTPN6, PTPN11
- Ligand/Receptor
- CD47, SP-A, SP-D
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
- Antigen References
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1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510. - Gene ID
- 19261 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD172a (SIRPα) Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
FITC anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... Fixed whole mount mouse spleen was stained with FITC CD172a ... -
PE/Cyanine7 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PerCP/Cyanine5.5 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
PE anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
APC anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PE/Dazzle™ 594 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Alexa Fluor® 594 anti-mouse CD172a (SIRPα)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Cyanine7 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Alexa Fluor® 488 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... Confocal image of C57BL/6 mouse thymus sample acquired using... Mice were injected subcutaneously with sheep red blood cells... -
Alexa Fluor® 700 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Biotin anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
Alexa Fluor® 647 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Fire™ 750 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
TotalSeq™-A0422 anti-mouse CD172a (SIRPα)
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Brilliant Violet 510™ anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
TotalSeq™-C0422 anti-mouse CD172a (SIRPα)
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Ultra-LEAF™ Purified anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
TotalSeq™-B0422 anti-mouse CD172a (SIRPα)
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PerCP/Fire™ 780 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark Red™ 718 anti-mouse CD172a (SIRPα) (Flexi-Fluor™)
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