- Clone
- 3.9 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III NL707
- Other Names
- Integrin αX subunit, CR4, p150, ITGAX
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3.9_LEAF_052412 3.9_LEAF_052412](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3.9_LEAF_052412.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes were stained with Ultra-LEAF™ purified CD11c (clone 3.9) (filled histogram) or LEAF™ purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
301631 | 100 µg | 237 CHF | ||||
301632 | 1 mg | 655 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
CyTOF® - Verified
IHC, Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). -
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
- Ottonello L, et al. 1999. Blood 93:3505.
- Metelitsa LS, et al. 2002. Blood 99:4166.
- Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
- Ihanus E, et al. 2007. Blood 109:802-810.
- Gurer C, et al. 2008. Blood 112:1231. PubMed
- Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
- RRID
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AB_2814120 (BioLegend Cat. No. 301631)
AB_2561480 (BioLegend Cat. No. 301632)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
- Distribution
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Myeloid, dendritic cells, NK cells, B cells and T cell subsets
- Function
- Adhesion, CTL killing
- Ligand/Receptor
- CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
- Cell Type
- B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810. - Gene ID
- 3687 View all products for this Gene ID
- UniProt
- View information about CD11c on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD11c Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD11c | 3.9 | FC |
PE anti-human CD11c | 3.9 | FC |
Purified anti-human CD11c | 3.9 | FC,CyTOF®,IHC |
PE/Cyanine7 anti-human CD11c | 3.9 | FC |
PE/Cyanine5 anti-human CD11c | 3.9 | FC |
Biotin anti-human CD11c | 3.9 | FC |
APC anti-human CD11c | 3.9 | FC |
Alexa Fluor® 488 anti-human CD11c | 3.9 | FC |
Alexa Fluor® 647 anti-human CD11c | 3.9 | FC |
Pacific Blue™ anti-human CD11c | 3.9 | FC |
PerCP/Cyanine5.5 anti-human CD11c | 3.9 | FC |
Brilliant Violet 421™ anti-human CD11c | 3.9 | FC |
Brilliant Violet 711™ anti-human CD11c | 3.9 | FC |
Ultra-LEAF™ Purified anti-human CD11c | 3.9 | FC,CyTOF®,Block,IHC |
Brilliant Violet 510™ anti-human CD11c | 3.9 | FC,ICC |
Brilliant Violet 605™ anti-human CD11c | 3.9 | FC |
Brilliant Violet 650™ anti-human CD11c | 3.9 | FC |
Purified anti-human CD11c (Maxpar® Ready) | 3.9 | FC,CyTOF® |
PE/Dazzle™ 594 anti-human CD11c | 3.9 | FC |
Brilliant Violet 785™ anti-human CD11c | 3.9 | FC |
Alexa Fluor® 700 anti-human CD11c | 3.9 | FC |
APC/Fire™ 750 anti-human CD11c | 3.9 | FC |
Spark Red™ 718 anti-human CD11c | 3.9 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
PE anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE -
Purified anti-human CD11c
Human peripheral blood granulocytes were stained with purifi... -
PE/Cyanine7 anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE/Cyan... -
PE/Cyanine5 anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE/Cyan... -
Biotin anti-human CD11c
Human peripheral blood granulocytes stained with biotinylate... -
APC anti-human CD11c
Human peripheral blood monocytes stained with 3.9 APC -
Alexa Fluor® 488 anti-human CD11c
Human peripheral blood monocytes stained with 3.9 Alexa Fluo... -
Alexa Fluor® 647 anti-human CD11c
Human peripheral blood monocytes stained with 3.9 Alexa Fluo... -
Pacific Blue™ anti-human CD11c
Human peripheral blood monocytes stained with anti-human CD1... -
PerCP/Cyanine5.5 anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... Human peripheral blood monocytes were stained with CD11c (cl... -
Brilliant Violet 421™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
Brilliant Violet 711™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
Ultra-LEAF™ Purified anti-human CD11c
Human peripheral blood granulocytes were stained with Ultra-... -
Brilliant Violet 510™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... Human peripheral blood mononuclear cell (PBMC) derived dendr... -
Brilliant Violet 605™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
Brilliant Violet 650™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
Purified anti-human CD11c (Maxpar® Ready)
-
PE/Dazzle™ 594 anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
Brilliant Violet 785™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c ... -
Alexa Fluor® 700 anti-human CD11c
Human peripheral blood monocytes were stained with Alexa Flo... Human peripheral blood lymphocytes were stained with PE anti... -
APC/Fire™ 750 anti-human CD11c
Human peripheral blood monocytes were stained with APC/Fire™... Human peripheral blood lymphocytes were stained with PE anti... -
Spark Red™ 718 anti-human CD11c
Human peripheral blood monocytes were stained with anti-huma... Human peripheral blood lymphocytes were stained with anti-hu...
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