- Clone
- NOK-1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VII 70322
- Other Names
- Fas Ligand (FasL), CD95L, TNFSF6, Fas-L
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human Fas Ligand transfected cells stained with LEAF™ purified NOK-1, followed by biotinylated anti-mouse IgG and Sav-PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
306415 | 100 µg | 220 CHF | ||||
306416 | 1 mg | 737 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- L5178Y mouse T lymphoma cells expressing recombinant human FasL
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
ICC, IP, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).
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Application References
(PubMed link indicates BioLegend citation) -
- Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
- Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
- Bossi G, et al. 1999. Nature Medicine 5:90.
- Andreola G, et al. 2002. J. Exp. Med. 195:1303.
- Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
- Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
- Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
- Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
- Qin G, et al. 2012. J. Infect. Dis. PubMed
- Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
- Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
- Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
- Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
- Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
- Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
- Product Citations
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- RRID
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AB_2810458 (BioLegend Cat. No. 306415)
AB_2810459 (BioLegend Cat. No. 306416)
Antigen Details
- Structure
- TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
- Distribution
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Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells
- Function
- Apoptosis, immune privilege
- Ligand/Receptor
- CD95
- Cell Type
- Neutrophils, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
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1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129. - Gene ID
- 356 View all products for this Gene ID
- UniProt
- View information about CD178 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD178 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-human CD178 (Fas-L) | NOK-1 | FC |
PE anti-human CD178 (Fas-L) | NOK-1 | FC |
Purified anti-human CD178 (Fas-L) | NOK-1 | FC,ICC,IP,WB |
Brilliant Violet 421™ anti-human CD178 (Fas-L) | NOK-1 | FC |
TotalSeq™-A0177 anti-human CD178 (Fas-L) | NOK-1 | PG |
Ultra-LEAF™ Purified anti-human CD178 (Fas-L) | NOK-1 | FC,ICC,IP,WB |
PE/Cyanine7 anti-human CD178 (Fas-L) | NOK-1 | FC |
TotalSeq™-C0177 anti-human CD178 (Fas-L) Antibody | NOK-1 | PG |
APC anti-human CD178 (Fas-L) | NOK-1 | FC |
TotalSeq™-B0177 anti-human CD178 (Fas-L) | NOK-1 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells stained with biotinylated... -
PE anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells stained with NOK-1 PE -
Purified anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells stained with purified NOK... -
Brilliant Violet 421™ anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells were stained with CD178 (... -
TotalSeq™-A0177 anti-human CD178 (Fas-L)
-
Ultra-LEAF™ Purified anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells stained with LEAF™ purifi... -
PE/Cyanine7 anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells were stained with CD178 (... -
TotalSeq™-C0177 anti-human CD178 (Fas-L) Antibody
-
APC anti-human CD178 (Fas-L)
Human Fas Ligand transfected cells were stained with CD178 (... -
TotalSeq™-B0177 anti-human CD178 (Fas-L)
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