- Clone
- MHR73-11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD180, Radio-protective 105-kD protein, RP105
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MHR73-11_LEAF_071607 MHR73-11_LEAF_071607](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MHR73-11_LEAF_071607.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified MHR73-11, followed by anti-mouse IgG FITC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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312913 | 100 µg | 264 CHF | ||||
312914 | 1 mg | 743 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human RP105 complexed with mouse MD-1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: intracellular staining1, immunoprecipitation1, immunohistochemistry1 of acetone-fixed frozen tissue sections, B cell activation1, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1)1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312913 and 312914).
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Application References
(PubMed link indicates BioLegend citation) -
- Miura Y, et al. 1998. Blood 92:2815.
- Indrevaer RL, et al. 2013. J. Immunol. 191:3624. PubMed
- Product Citations
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- RRID
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AB_2876614 (BioLegend Cat. No. 312913)
AB_2876614 (BioLegend Cat. No. 312914)
Antigen Details
- Structure
- Member of the toll-like receptor family, 105 kD
- Distribution
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B cells, monocytes/macrophages, and dendritic cells
- Function
- Signaling protein involved in innate immunity
- Ligand/Receptor
- LPS
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
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1. Miura Y, et al. 1998. Blood 92:2815.
- Gene ID
- 4064 View all products for this Gene ID
- UniProt
- View information about CD180 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD180 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human CD180 (RP105) | MHR73-11 | FC,ICFC,IHC-F,IP |
PE anti-human CD180 (RP105) | MHR73-11 | FC |
PE/Cyanine7 anti-human CD180 (RP105) | MHR73-11 | FC |
APC anti-human CD180 (RP105) | MHR73-11 | FC |
Ultra-LEAF™ Purified anti-human CD180 (RP105) | MHR73-11 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with purified MHR... -
PE anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with MHR73-11 PE -
PE/Cyanine7 anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with CD180 (clone... -
APC anti-human CD180 (RP105)
Human peripheral blood lymphocytes were stained with CD180 (... -
Ultra-LEAF™ Purified anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ...
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