- Clone
- A15153G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_A15153G_ULEAF_TIGIT_Antibody_1_032817 1_A15153G_ULEAF_TIGIT_Antibody_1_032817](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15153G_ULEAF_TIGIT_Antibody_1_032817.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood leukocytes were stained with CD3 FITC and Ultra-LEAF purified anti-human TIGIT (clone A15153G) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by PE goat anti-mouse IgG. Data shown were gated on lymphocyte population. -
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Human PBMCs were labeled with CFSE. Cells were cultured in 6-well plate wells that were pre-coated with anti-CD3 plus mouse IgG2a isotype control (open histogram) or with anti-CD3 plus anti-human TIGIT (clone A15153G) (filled histogram). After four days of incubation, cells were harvested and stained for surface CD3. Data shown are SFSE profiles of CD3+ cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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372719 | 100 µg | 209 CHF | ||||
372720 | 1 mg | 572 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human TIGIT.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
Additional reported applications (for the relevant formats) include: Blocking1. - Additional Product Notes
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View more applications data for this product in our Scientific Poster Library.
Watch a Scientific Poster video of this product. -
Application References
(PubMed link indicates BioLegend citation) -
- Stamm H, et al. 2018. Oncogene. Pubmed
- Product Citations
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- RRID
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AB_2650966 (BioLegend Cat. No. 372719)
AB_2650967 (BioLegend Cat. No. 372720)
Antigen Details
- Structure
- 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
- Distribution
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Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
- Function
- Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
- Ligand/Receptor
- CD155 (PVR), CD112 (PVRL2, NECTIN-2).
- Cell Type
- NK cells, T cells, Tfh, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
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1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923. - Gene ID
- 201633 View all products for this Gene ID
- UniProt
- View information about TIGIT on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All TIGIT (VSTM3) Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... Human PBMCs were labeled with CFSE. Cells were cultured in ... -
APC/Fire™ 750 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... -
APC anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... -
PE anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 APC ... -
Brilliant Violet 421™ anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... -
Brilliant Violet 605™ anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... -
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... -
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 APC ... -
PE/Cyanine7 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with FITC CD3... -
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... Human PBMCs were labeled with CFSE. Cells were cultured in ... -
Biotin anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... -
Alexa Fluor® 647 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 PE ... -
TotalSeq™-A0089 anti-human TIGIT (VSTM3)
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TotalSeq™-B0089 anti-human TIGIT (VSTM3)
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TotalSeq™-C0089 anti-human TIGIT (VSTM3)
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KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 APC... -
APC/Cyanine7 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 PE ... -
Brilliant Violet 510™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody
Human peripheral blood lymphocytes were stained with CD3 PE ... -
TotalSeq™-D0089 anti-human TIGIT (VSTM3)
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Brilliant Violet 711™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 Ale... -
PE/Fire™ 640 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with anti-hum... -
PE/Fire™ 810 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocyte were stained with anti-hum... -
PE/Cyanine5 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with anti-hum... -
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)
Human lysed whole blood cells were stained with anti-human C... -
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)
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PE/Fire™ 700 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with anti-hu...
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