- Clone
- HTK888 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Trinitrophenol + KLH
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC, ICFC - Quality tested
ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg). -
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_11203529 (BioLegend Cat. No. 400960)
AB_11203529 (BioLegend Cat. No. 400968)
AB_11203529 (BioLegend Cat. No. 400967)
AB_11203529 (BioLegend Cat. No. 400969)
AB_11203529 (BioLegend Cat. No. 400940)
AB_11203529 (BioLegend Cat. No. 400959)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Armenian Hamster IgG Isotype Ctrl
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Biotin Armenian Hamster IgG Isotype Ctrl
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FITC Armenian Hamster IgG Isotype Ctrl
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PE Armenian Hamster IgG Isotype Ctrl
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PE/Cyanine5 Armenian Hamster IgG Isotype Ctrl
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Purified Armenian Hamster IgG Isotype Ctrl
Whole cell extracts (190 µg total protein) prepared from Jur... Chromatin Immunoprecipitations (ChIP) were done with fixed a... -
PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 647 Armenian Hamster IgG Isotype Ctrl
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Pacific Blue™ Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 700 Armenian Hamster IgG Isotype Ctrl
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APC/Cyanine7 Armenian Hamster IgG Isotype Ctrl
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PerCP Armenian Hamster IgG Isotype Ctrl
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PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 570™ Armenian Hamster IgG Isotype Ctrl
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Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 510™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 605™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 650™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 785™ Armenian Hamster IgG Isotype Ctrl
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PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 594 Armenian Hamster IgG Isotype Ctrl
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APC/Fire™ 750 Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 711™ Armenian Hamster IgG Isotype Ctrl
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GoInVivo™ Purified Armenian Hamster IgG Isotype Ctrl
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TotalSeq™-A0241 Armenian Hamster IgG Isotype Ctrl
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KIRAVIA Blue 520™ Armenian Hamster IgG Isotype Ctrl
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TotalSeq™-C0241 Armenian Hamster IgG Isotype Ctrl
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TotalSeq™-B0241 Armenian Hamster IgG Isotype Ctrl
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Spark YG™ 570 Armenian Hamster IgG Isotype Ctrl
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Spark Red™ 718 Armenian Hamster IgG Isotype Ctrl
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Spark Blue™ 515 Armenian Hamster IgG Isotype Ctrl
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PerCP/Fire™ 780 Armenian Hamster IgG Isotype Ctrl
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Spark Blue™ 574 Armenian Hamster IgG Isotype Ctrl
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Spark PLUS UV™ 395 Armenian Hamster IgG Isotype Ctrl
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