- Clone
- K13/16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- C3a, C3a desarg, C3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![C3a_040511 C3a_040511](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/C3a_040511.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
518105 | 100 µg | 270 CHF | ||||
518106 | 1 mg | 743 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
C3, complement component 3, is a 195 kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human C3a
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA Capture - Quality tested
IP, WB, Neut - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from 39 to 2500 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The K13/16 antibody reacts with human C3a, C3a(desArg) and C3. The K13/16 antibody can neutralize the bioactivity of natural or recombinant C3a.
ELISA Capture: The purified K13/16 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated D17/1 antibody (Cat. No. 518002) as the detecting antibody.
Neutralization: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human C3a/C3a(desArg)/C3 bioactivity. -
Application References
(PubMed link indicates BioLegend citation) -
- Oppermann M, et al. 1991. Kidney Int. 40:939947.
- Oppermann M, et al. 1992. Immunopharm. 24:119134.
- Elsner J, et al. 1994. Blood 83:3324.
- Product Citations
-
- RRID
-
AB_2861044 (BioLegend Cat. No. 518105)
AB_2861045 (BioLegend Cat. No. 518106)
Antigen Details
- Ligand/Receptor
- C3a bind to C3a receptor, C3a desArg bind to C5L2 (GPR77)
- Biology Area
- Cell Biology, Complement, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
1. de Bruijn MH, et al. 1985. P. Natl. Acad. Sci. USA 82:708.
2. Hugli TE, et al. 1975. J. Biol. Chem. 250:8293.
3. Oxvig C, et al. 1995. J. Biol. Chem. 270:13645. - Gene ID
- 718 View all products for this Gene ID
- UniProt
- View information about C3a/C3adesArg/C3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All C3a/C3a(desArg)/C3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human C3a/C3a(desArg)/C3 | K13/16 | ELISA Capture,IP,WB |
Ultra-LEAF™ Purified anti-human C3a/C3a(desArg)/C3 | K13/16 | ELISA Capture,IP,WB,Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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