- Clone
- K28/74 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Disks large homolog 4, discs large homolog 4, Tax interaction protein 15, synapse-associated protein 90, postsynaptic density protein 95, post-synaptic density protein 95
- Previously
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Covance Catalog# MMS-5183
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_K28slash74_PURE_PSD95_WB_021218 A_K28slash74_PURE_PSD95_WB_021218](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/K28slash74_PURE_PSD95_WB_021218.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-PSD95 antibody (clone K28/74). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
810302 | 25 µL | 83 CHF | ||||
810301 | 100 µL | 165 CHF |
PSD95, also known as SAP-90, is a member of the MAGUK family, encoded by the the DLG4 gene. At postsynaptic sites, it mediates the scaffolding of NMDA receptors, AMPA receptors, potassium channels, and other associated signaling protein clusters. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 5.0 µg/ml. For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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- RRID
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AB_2734563 (BioLegend Cat. No. 810302)
AB_2564749 (BioLegend Cat. No. 810301)
Antigen Details
- Structure
- Expected MW: 95-110 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 1742 View all products for this Gene ID
- UniProt
- View information about PSD95 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All PSD95 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PSD95 | K28/74 | WB,IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-PSD95
Western blot of purified anti-PSD95 antibody (clone K28/74).... IHC staining of purified anti-PSD95 antibody (clone K28/74) ... IHC staining of purified anti-PSD95 antibody (clone K28/74) ... IHC staining of purified anti-PSD95 antibody (clone K28/74) ... IHC staining of purified anti-PSD95 antibody (clone K28/74) ... IHC staining of purified anti-PSD95 antibody (clone K28/74) ...
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