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| BioLegend is constantly innovating on the design and quality of our reagents. Our latest buffer, the True-Nuclear™ Transcription Factor Buffer Set, offers improved staining for nuclear factor targets. This buffer can now also be found in our new Treg staining kits and provides outstanding and improved staining for FOXP3, a Treg transcription factor vital to T regulatory cell function and development. Whether staining for FOXP3, Helios, Blimp-1, or T-bet, True-Nuclear™ is an excellent choice for distinct and effective nuclear factor staining. |
True-Nuclear™ Human Treg Flow™ Kits |
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| Human peripheral blood lymphocytes were stained with True-Nuclear™ Human Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD4 PE-Cy5/CD25 PE). |
True-Nuclear™ Transcription Factor Buffer Set Staining |
| PE/Dazzle™ 594 anti-mouse/human Helios Antibody | Brilliant Violet 421™ anti-human FOXP3 Antibody |
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| Human peripheral blood lymphocytes were surface stained with CD4 Alexa Fluor® 647 and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Helios (clone 22F6) PE/Dazzle™ 594. | Human peripheral blood lymphocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with FOXP3 (clone 206D) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right). |
Related Products |
| Buffers |
| Description |
|---|
| Flow Cytometry Kits |
| Description | FOXP3 Clone |
|---|---|
| True-Nuclear™ Human Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD4 PE-Cy5/CD25 PE) | 150D | 259D |
| True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD25 PE/CD4 PerCP) | MF-14 |
| Human Antibodies |
| Description | Clone |
|---|---|
| FOXP3 | 150D | 206D | 259D |
| Helios | 22F6 |
| T-bet | 4B10 |
| Mouse Antibodies |
| Description | Clone |
|---|---|
| Blimp-1 | 5E7 |
| FOXP3 | 150D | MF-14 |
| Helios | 22F6 |
| T-bet | 4B10 |
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