- Regulatory Status
- RUO
- Other Names
- Leucine-rich repeat-containing G-protein coupled receptor 5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Human_LGR5_Fc_CF_RECOM_1_011720 Human_LGR5_Fc_CF_RECOM_1_011720](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Human_LGR5_Fc_CF_RECOM_1_011720.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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When recombinant human R-Spondin 1 (Cat. No. 783602) is immobilized at 2 µg/mL, recombinant human LGR5-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 15 - 60 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding. -
Stability testing for recombinant human LGR5-Fc Chimera. Recombinant human LGR5-Fc Chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with human R-Spondin 1.
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LGR5 (leucine-rich repeat G-protein coupled receptor 5, also known as GPR49) is a member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The LGR subfamily consists of LGR4, LGR5, and LGR6 which are G-protein independent mediators of the R-Spondin effect on Wnt signaling. LGR5 binds R-Spondins to form complexes with Frizzled Wnt receptors and LRP Wnt co-receptors. It potentiates the R-Spondin effect by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of β-catenin. In contrast to classical G-protein coupled receptors, LGR5 does not activate heterotrimeric G-proteins to transduce the signal. LGR5 is expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain. It is also expressed at the base of crypts in colonic and small mucosa stem cells. LGR5 plays a role in the formation and maintenance of adult intestinal stem cells during post-embryonic development. Several transcript variants encoding different isoforms have been found for LGR5. In mice, Lgr5+ stem cells are sufficient to produce all epithelial cell types of the intestinal crypts in vivo or in vitro. LGR5 is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas, and may promote carcinogenesis.
Product DetailsProduct Details
- Source
- Human LGR5, amino acid Gly22-Ile560 (Accession # O75473), with a C-terminal hIgG1 Fc tag, was expressed in CHO cells.
- Molecular Mass
- The 778 amino acid recombinant protein has a predicted molecular mass of approximately 87.1 kD. The DTT-reduced protein migrates at approximately 90 - 110 kD and and the non-reduced protein migrates at approximately 180 - 220 kD by SDS-PAGE. The predicted N-terminal amino acid is Gly.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 1X PBS, pH 7.2, 0.5 M NaCl, 10% Glycerol.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human R-Spondin 1 (Cat. No. 783602) is immobilized at 2 µg/mL, recombinant human LGR5-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 15 - 60 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Antigen Details
- Structure
- Homodimer
- Distribution
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LGR5 is expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain. It is also expressed at the base of crypts in colonic and small mucosa stem cells.
- Function
- Receptor for R-spondins that potentiates the canonical Wnt signaling pathway, and acts as a stem cell marker of the intestinal epithelium and the hair follicle
- Interaction
- LRP6, Frizzled receptors
- Ligand/Receptor
- RSPO1, RSPO2, RSPO3
- Bioactivity
- Measured by its ability to bind recombinant human R-Spondin 1 (Cat. No. 783602)
- Biology Area
- Cancer Biomarkers, Stem Cells
- Molecular Family
- Soluble Receptors
- Antigen References
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1. Barker N, et al. 2007. Nature. 449:1003-7.
2. Glinka A, et al. 2011. EMBO Rep. 12:1055-61.
3. de Lau W, et al. 2011. Nature. 476:293-7.
4. Carmon KS, et al. 2011. Proc Natl Acad Sci U S A. 108:11452-7.
5. Ruffner H, et al. 2012. PLoS ONE. 7:E40976.
6. Peng WC, et al. 2013. Cell Rep. 3:1885-92.
- Gene ID
- 8549 View all products for this Gene ID
- UniProt
- View information about LGR5 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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