LEGENDplex™ Human Th1 Panel (5-plex) with Filter Plate

Pricing & Availability
Other Names
Hu Th Cytokine Panel
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Save
740723 100 tests 560€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

T helper (Th) cells play an important role in regulating immune reponses. They secrete cytokines to stimulate various effector cells, such as cytotoxic T cells, B cells, and macrophages. Accurate measurement of Th cytokine expression is critical to identify the corresponding Th cells and for in-depth understanding of the immune responses.


The Human Th1 Panel is a multiplex bead-based assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of five human cytokines, including IL-2, IL-6, IL-10, IFN-γ and TNF-α, which are secreted by Th1 cells. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum and cell culture supernatant samples. Use caution when using plasma samples due to compromised accuracy in these samples.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads 1: FITC Beads (1 ml)
  • Setup Beads 2: PE Beads (1 ml)
  • Setup Beads 3: Raw Beads (2 ml)
  • Capture Beads (varies, see manual)
  • Detection Antibodies (3.5 ml)
  • Standard Cocktail, Lyophilized (1 vial)
  • SA-PE (3.5 ml)
  • Matrix B, Lyophilized (1 vial)
  • Assay Buffer (25 ml)
  • Wash Buffer, 20X (25 ml)
  • Plate Sealers (4 sheets)
  • Data Analysis Software Dongle
  • Filter Plate
  • Manual

Product Details

Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Vortex Mixer
  • Sonicator 
  • Aluminum Foil
  • Absorbent Pads or Paper Towels
  • Plate Shaker
  • Tabletop Centrifuges
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
  • 1.1 mL polypropylene micro FACS tubes, in 96-tube rack

Related FAQs

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

If I don't have a vacuum, how do I remove the liquid from my plate?

Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are valid for 6 months from the date of receipt. 

Go To Top Version: 1    Revision Date: 05/04/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account