Purified anti-Aph-1a, 245-265 (C terminus) Antibody (Previously Covance catalog# PRB-550P)

Pricing & Availability
Clone
Poly18231 (See other available formats)
Other Names
Gamma-secretase subunit APH-1A, aph-1alpha, presenilin-stabilization factor, CGI-78, anterior pharynx defective 1 homolog A, presenilin-stabilization factor
Previously
Covance Catalog# PRB-550P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
823101 100 µl 305€
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Description

The gamma secretase is a multiprotein complex consisting of presenilin, nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. While Aph-1 stabilizes the presenilin holoprotein within the gamma secretase complex, Pen-2 is required for endoproteolysis of presenilin and activation of the gamma secretase. The Aph-1 homologue on human chromosome 1 is termed Aph-1a and on human chromosome 15 is referred to as Aph-1b.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The antibody Aph-1a, 245-265 (Poly18231) was raised against the human Aph-1aL/C-terminal region and recognizes the sequence CRRQEDSRVMVYSALRIPPED of human Aph-1aL.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IF

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000*

Application Notes

This antibody is effective in immunoblotting (WB).

*Expected MW: 29 kD

WB Positive Control: Human Brain lysate

BioLegend also offers Poly18232 (823201) which recognizes residues 92-115 of the Aph-1aL/ S Loop.


This product may contain other non-IgG subtypes.

Application References
  1. Gu Y, Chen F, Sanjo N, Kawarai T, Hasegawa H, Duthie M, Li W, Ruan X, Luthra A, Mount HT, Tandon A, Fraser PE, St George-Hyslop P. Aph-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of the presenilin.nicastrin complexes. J Biol Chem 278:7374-7380, 2003.
Product Citations
  1. Lu J, et al. 2016. PLoS One. 11:e0166415. PubMed
Publication Library
RRID
AB_2564862 (BioLegend Cat. No. 823101)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Secretases
Gene ID
51107 View all products for this Gene ID
UniProt
View information about Aph-1a, 245-265 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/09/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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