Purified anti-EPO Antibody

Pricing & Availability
Clone
M029F6 (See other available formats)
Other Names
Erythropoietin
Isotype
Rat IgG2b
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Product Citations
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M029F6_Purified_EPO_Antibody_WB_043014.jpg
Western blot analysis of 50 ng of recombinant human EPO (lane 1) and 50 ng of recombinant mouse EPO (lane 2) using anti-mouse EPO antibody (clone M029F6).
  • M029F6_Purified_EPO_Antibody_WB_043014.jpg
    Western blot analysis of 50 ng of recombinant human EPO (lane 1) and 50 ng of recombinant mouse EPO (lane 2) using anti-mouse EPO antibody (clone M029F6).
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660302 100 µg 196€
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Description

EPO is a glycoprotein that is glycosylated up to 40-60%, which causes its molecular weight to vary from 30-34 kD depending on the carbohydrate percentage. Hypoxia induces erythropoiesis, and hypoxia inducible factor (HIF) is directly involved in EPO expression. HIF-Prolyl hydroxylase (HIF-PH) is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induces EPO production in the kidneys and liver, and mobilizes iron to support erythropoiesis. EPO has been used in the treatment of anemia associated with chronic kidney disease, treatment of cancer patients on chemotherapy, and antiviral HIV therapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse EPO
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not cross react with human EPO.

RRID
AB_2563470 (BioLegend Cat. No. 660302)

Antigen Details

Structure
166 amino acid, predicted molecular weight of approximately 18.6 kD, migrates at approximately 30-34 kD as a result of its glycosylation.
Distribution
EPO is primarily expressed in the kidneys (70-90% of the total EPO) and liver. EPO is expressed by hepatocytes, cortical interstitial fibroblasts in ischemic kidneys, astrocytes, and neurons under hypoxia.
Function
EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. EPO expression is induced by hypoxia, HIF, and other transcription factors. EPO has a possible role in neuroprotection.
Interaction
Erythroid progenitor cells.
Ligand/Receptor
EpoR (homodimer).
Cell Type
Fibroblasts, Neurons
Biology Area
Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Shoemaker CB, et al. 1986. Mol. Cell. Biol. 6:849.
2. McDonald JD, et al. 1986. Mol. Cell. Biol. 6:842.
3. Chin K, et al. 2000. Brain Res. Mol. Brain Res. 81:29.
4. Snow JW, et al. 2009. J. Biol. Chem. 284:29310.
5. Kassouf MT, et al. 2010. Genome Res. 2010:20:1064.
6. Paliege A, et al. 2010. Kidney Int. 77:312.
7. Sinclair AM, et al. 2010. Blood 115:4264.
8. Alnaeeli M, et al. 2012. Anat. Res. Int. 2012:953264.

Gene ID
2056 View all products for this Gene ID
UniProt
View information about EPO on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/15/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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