Purified anti-NR1D2 Antibody

Pricing & Availability
Clone
W15136A (See other available formats)
Other Names
Nuclear receptor subfamily 1 group D member 2, BD73, V-erbA-related protein 1-related (EAR-1r), Hs.37288, HZF2, Rev-erb alpha-related receptor (RVR), Rev-erb-beta
Isotype
Rat IgG1, κ
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W15136A_Purified_NR1D2_Antibody_1_WB_082616
Total cell lysate from human 293T cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-NR1D2 (clone W15136A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-Tubulin-α (clone 10D8) antibody was used as a loading control.
  • W15136A_Purified_NR1D2_Antibody_1_WB_082616
    Total cell lysate from human 293T cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-NR1D2 (clone W15136A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-Tubulin-α (clone 10D8) antibody was used as a loading control.
  • W15136A_Purified_NR1D2_Antibody_2_ICC_041519
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 1.0 µg/ml purified anti- NR1D2 (clone W15136A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-rat IgG antibody (red) for one hour at room temperature. Actin filaments were labelled with Alexa Fluor® 488 conjugated Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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689402 100 µg 360€
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Description

Nuclear receptor subfamily 1, group D, member 2 (NR1D2), also known as Rev-ErbA β, is a member of the nuclear hormone receptor family. This protein can modulate gene expression by directly binding to DNA response elements within the promotor of its target genes as either monomers or homodimers. NR1D2 is well known to function as a transcriptional repressor. By complexing with heme, NR1D2 coordinates circadian rhythm and controls lipid metabolism. NR1D2 can directly repress the expression of core clock components ARNTL/BMAL1 and CLOCK, to coordinate circadian rhythm. In addition, NR1D2 can regulate lipid and energy homeostasis in skeletal muscles via repression of lipid metabolism and myogenesis-related genes such as CD36, FABP, SCD1 and MSTN. It is also involved in the regulation of hepatic lipid metabolism through the suppression of APOC3 expression. Not only as a repressor, NR1D2 also acts as a transcriptional activator; it can activate the transcription of the sterol regulatory element-binding protein 1 (STEBF1) and the inflammatory mediator IL-6 in skeletal muscle. Alternatively spliced transcript variants of NR1D2 gene have been described.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human NR1D2 recombinant protein (196-399 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629653 (BioLegend Cat. No. 689402)

Antigen Details

Structure
579 amino acids, predicted molecular weight of 65 kD. Contains two N-terminal zinc finger domains responsible for DNA binding.
Distribution

Nucleus.

Function
NR1D2 belongs to the nuclear hormone receptor family and acts as a transcriptional repressor. NR1D2 participates in the regulation of circadian rhythms and energy homeostasis.
Interaction
Forms a DNA-binding homodimer. Interacts with NCOA5, KAT5, HDAC1, and ZNHIT1.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers, Steroid Receptors/Nuclear Receptors
Antigen References

1. Cho H, et al. 2012. Nature 485:123.
2. Gupta N, Ragsdale SW. 2011. J. Biol. Chem. 286:4392.
3. Bois-Joyeux B, et al. 2000. DNA Cell Biol. 19:589-99.
4. Burke L, et al. 1996. Nucleic Acids Res. 24:3481.
5. Giambiagi N, et al. 1995. Biochem. Mol. Biol. Int. 37:1091.
6. Bonnelye E, et al. Cell Growth Differ. 5:1357.

Gene ID
9975 View all products for this Gene ID
UniProt
View information about NR1D2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/26/2016

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