- Clone
- 5B2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CEACAM1,5,6,8
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5B2_PURE_CD66a-b-c-e_Antibody_1_111519.png 5B2_PURE_CD66a-b-c-e_Antibody_1_111519.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5B2_PURE_CD66a-b-c-e_Antibody_1_111519.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66a/b/c/e (clone 5B2) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software. -
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66a/b/c/e (clone 5B2) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software. -
Human colorectal adenocarcinoma cell line HT-29 stained with CD66a/b/c/e (clone 5B2) purified (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) purified (open histogram) followed by anti-mouse IgG PE.
The 5B2 monoclonal antibody reacts with the antigen epitopes shared by CD66a, b, c and e, which are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neisseria, Moraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
FC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2820072 (BioLegend Cat. No. 398902)
Antigen Details
- Structure
- Ig superfamily, CEA antigen group, GPI-linked glycoprotein
- Distribution
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Granulocytes, epithelial cells
- Function
- Homophilic and heterophilic adhesion
- Ligand/Receptor
- CD66a-e, CD62E, galectins
- Cell Type
- Epithelial cells, Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
- Chen T, et al. 2001. J Biol Chem. 276:17413.
- Wang Z, et al. 2016. Mol Clin Oncol. 5:731.
- Swords DS, et al. 2016. Onco Targets Ther. 9:7459
- Gene ID
- 1088 View all products for this Gene ID 634 View all products for this Gene ID 4680 View all products for this Gene ID 1048 View all products for this Gene ID
- UniProt
- View information about CD66a/b/c/e on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD66a/b/c/e Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD66a/b/c/e | 5B2 | IHC-P,FC |
Alexa Fluor® 647 anti-human CD66a/b/c/e Antibody | 5B2 | FC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD66a/b/c/e
Human paraffin-embedded colon tissue slices were prepared wi... Human paraffin-embedded spleen tissue slices were prepared w... Human colorectal adenocarcinoma cell line HT-29 stained with... -
Alexa Fluor® 647 anti-human CD66a/b/c/e Antibody
Human colorectal adenocarcinoma cell line were stained with ... Human paraffin-embedded colon tissue slices were prepared wi... Human paraffin-embedded spleen tissue slices were prepared w...
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