- Clone
- 2B10B19 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Octamer-binding protein 2, Octamer-binding transcription factor 2 (OTF-2), POU domain, class 2, transcription factor 2 (POU2F2)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2B10B19_PURE_OCT2_Antibody_0_WB_120718 2B10B19_PURE_OCT2_Antibody_0_WB_120718](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2B10B19_PURE_OCT2_Antibody_0_WB_120718.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from HeLa and Molt-4 (reduced expression controls), Daudi, and A20 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-OCT2 Antibody, clone 2B10B19, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. -
Total cell lysate from human Ramos cells (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 2µg per mL of purified monoclonal anti-OCT2 (clone 2B10B19) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-Tubulin-α (clone 10D8) antibody was used as a loading control.Â
Cat # | Size | Price | Save |
---|---|---|---|
659801 | 25 µg | ¥22,220 | |
659802 | 100 µg | ¥58,300 |
OCT2 is a member of the POU family of transcription factors that was found to be expressed predominantly in B lymphocytes, activated T lymphocytes, and neuronal cells. OCT2 binds to the conserved octamer DNA element, ATGCAAAT, which was discovered in the promoters and enhancers of immunoglobulin (Ig) genes. In addition to regulating Ig gene expression, OCT2 controls several B cell specific genes required for proliferation and differentiation, such as CD20, CD36, and CD79a. OCT2 was found to have different isoforms produced by alternative splicing. The OCT2 isoforms exert distinct functional activities and can act as transcriptional activators or repressors.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human OCT2 recombinant protein (1-200 aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone may produce a non-specific band of ~75 kD by western blot.
- RRID
-
AB_2563348 (BioLegend Cat. No. 659801)
AB_2563350 (BioLegend Cat. No. 659802)
Antigen Details
- Structure
- 479 amino acids, predicted molecular weight of 51 kD (isoform 1). Contains a POU-specific domain and a Homeobox DNA-binding domain.
- Distribution
-
Expressed predominantly in B cells, activated T cells, and neurons.
- Function
- OCT2 is a transcription factor, regulating the transactivation of B cell-specific genes involved in immunoglobulin production, B cell differentiation, and proliferation.
- Interaction
- Interacts with OBF-1, NR3C1, AR and PGR.
- Cell Type
- B cells, Neurons, T cells
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Heckman CA, et al. 2006. Oncogene. 25:888.
2. García-Cosío M, et al. 2004. Mod. Pathol. 17:1531.
3. Corcoran LM, et al. 2001. J. Immunol. 172:2962.
4. Shore P, et al. 2002. Nucleic Acids Res. 30:1767.
5. Corcoran L, et al. 2002. Int. Immunol. 14:1099.
6. Latchman DS. 1996. Int. J. Biochem. Cell Biol. 28:1081.
7. Kemler I, et al. 1990. FASEB J. 4:1444. - Gene ID
- 5452 View all products for this Gene ID
- UniProt
- View information about OCT2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All OCT2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-OCT2 | 2B10B19 | WB |
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