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Purified Streptavidin

Pricing & Availability
Regulatory Status
RUO
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
280302 250 µg $42
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Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical data sheet

Product Details

Formulation
Phosphate-buffered solution containing 0.09% sodium azide.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage

To coat one 96-well plate dilute 24 µL of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat. No. 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg/mL. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.

Product Citations
  1. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  2. Wu C, et al. 2020. J Am Chem Soc. 142:12314. PubMed

Antigen Details

Structure
52.8 kD tetrameric protein.
Distribution

Streptavidin binds to biotin with high affinity.

Ligand/Receptor
Biotin.
Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID
NA

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Go To Top Version: 3    Revision Date: 12/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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