- Clone
- A16075D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transmembrane protein 119
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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A16075D_A-488_-TMEM119_ICFC_070924 A.
A16075D_A-488_-TMEM119_ICFC_070924](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A16075D_A-488_-TMEM119_ICFC_070924-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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CHO cells untransfected (negative control, open histogram), or transiently transfected with a human TMEM119 expression vector (positive control, two peaks are observed which represent cells which have not or have successfully incorporated the TMEM119 expression vector, filled histogram). Samples were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 488 anti-TMEM119 (clone A16075D) or Alexa Fluor® 488 Mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for non-transfected or transfected cells) (Cat. No. 400329). -
Non-transfected CHO cells (negative control, panel A) or cells transiently transfected with a human TMEM119 expression vector (positive control, panel B) were fixed with PFA Fixation Buffer (Cat. No. 420801), permeabilized with methanol, and blocked with 5% FBS. Cells were stained with Alexa Flour® 488 anti-TMEM119 (clone A16075D). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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853317 | 25 µg | 144€ | ||||
853318 | 100 µg | 340€ |
Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human TMEM119 protein (extracellular domain)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron.
*The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.
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Application References
(PubMed link indicates BioLegend citation) -
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Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed
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Antigen Details
- Structure
- TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
- Distribution
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Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues.
Cellular distribution: Membrane and endoplasmic reticulum. - Function
- TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
- Interaction
- TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Antigen References
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- Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113
- Satoh J, et al. 2016. Neuropathology. 36:39-49
- Kawao N and Kaji H. 2015. J Cell Biochem. 116:687
- Zrzavy T, et al. 2017. Brain. 140:1900-1913
- Gene ID
- 338773 View all products for this Gene ID
- UniProt
- View information about TMEM119 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All TMEM119 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TMEM119 (Extracellular) | A16075D | FC,WB,ICC,IHC-P |
TotalSeq™-A0445 anti-TMEM119 (Extracellular) | A16075D | PG |
TotalSeq™-B0445 anti-TMEM119 (Extracellular) | A16075D | PG |
TotalSeq™-C0445 anti-TMEM119 (Extracellular) | A16075D | PG |
Alexa Fluor® 594 anti-TMEM119 (Extracellular) | A16075D | IHC-P |
Alexa Fluor® 647 anti-TMEM119 (Extracellular) | A16075D | ICFC,IHC-P,ICC |
PE anti-TMEM119 (Extracellular) | A16075D | ICFC |
Alexa Fluor® 488 anti-TMEM119 (Extracellular) | A16075D | ICFC,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-TMEM119 (Extracellular)
Human Embryonic Kidney 293 (HEK293) cells transfected with h... Western blot of anti-TMEM119 (Extracellular) antibody (clone... ICC staining of anti-TMEM119 (Extracellular) antibody (clone... IHC staining of purified anti-TMEM119 antibody (clone A16075... IHC staining of purified anti-TMEM119 antibody (A16075D) on ... -
TotalSeq™-A0445 anti-TMEM119 (Extracellular)
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TotalSeq™-B0445 anti-TMEM119 (Extracellular)
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TotalSeq™-C0445 anti-TMEM119 (Extracellular)
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Alexa Fluor® 594 anti-TMEM119 (Extracellular)
IHC staining of Alexa Flour® 594 anti-TMEM119 (clone A16075D... -
Alexa Fluor® 647 anti-TMEM119 (Extracellular)
Non-transfected RBL-1 cells (negative control, open histogra... IHC staining of Alexa Flour® 647 anti-TMEM119 (clone A16075D... Non- transfected RBL-1 cells (negative control, panel A) and... -
PE anti-TMEM119 (Extracellular)
Non-transfected RBL-1 cells (negative control, open histogra... -
Alexa Fluor® 488 anti-TMEM119 (Extracellular)
CHO cells untransfected (negative control, open histogram), ... Non-transfected CHO cells (negative control, panel A) or cel...
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