- Clone
- H4B4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- LAMPb, LGP110
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![H4B4_A594_CD107b_Antibody_101717 H4B4_A594_CD107b_Antibody_101717](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/H4B4_A594_CD107b_Antibody_101717.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD107b (clone H4B4, red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
354309 | 25 µg | 128€ | ||||
354310 | 100 µg | 306€ |
CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines. LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Adult human adherent spleen cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of neutrophils2,3.
This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation. -
Application References
(PubMed link indicates BioLegend citation) -
- Chen J, et al. 1985. J. Biol. Chem. 101:85.
- Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
- Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
- Product Citations
-
- RRID
-
AB_2721377 (BioLegend Cat. No. 354309)
AB_2721378 (BioLegend Cat. No. 354310)
Antigen Details
- Structure
- LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
- Distribution
-
Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines
- Function
- Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
- Cell Type
- B cells, Lymphocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538. - Gene ID
- 3920 View all products for this Gene ID
- UniProt
- View information about CD107b on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD107b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD107b (LAMP-2) | H4B4 | ICFC |
PE anti-human CD107b (LAMP-2) | H4B4 | ICFC |
Purified anti-human CD107b (LAMP-2) | H4B4 | ICFC,IHC-P,ICC,WB |
Alexa Fluor® 594 anti-human CD107b (LAMP-2) | H4B4 | IHC-P |
Alexa Fluor® 647 anti-human CD107b (LAMP-2) | H4B4 | ICFC,IHC-P |
Brilliant Violet 421™ anti-human CD107b (LAMP-2) | H4B4 | ICFC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... -
PE anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... -
Purified anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... Human paraffin-embedded prostate tissue slice was stained wi... ICC staining of purified anti-LAMP-2 antibody (clone H4B4) o... Western blot of purified anti-LAMP-2 antibody (clone H4B4). ... -
Alexa Fluor® 594 anti-human CD107b (LAMP-2)
Human paraffin-embedded prostate tissue slices were prepared... -
Alexa Fluor® 647 anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... Human paraffin-embedded prostate tissue slice were prepared ... -
Brilliant Violet 421™ anti-human CD107b (LAMP-2)
Human paraffin‐embedded prostate tissue slices were prepared... Human acute myeloid leukemia cell line KG1a was fixed, perme...
Follow Us