LEGEND MAX™ Human IL-12 (p70) ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-12 Pre-coated ELISA
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
431707 1 Pre-coated Plate 328€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-12 is produced by dendritic cells, macrophages, and human B-lymphoblastoid cells in response to antigenic stimulation. It is a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. IL-12 is involved in the differentiation of naïve T cells into Th0 cells which will further develop into either Th1 cells or Th2 cells. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. It mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-12 is linked with autoimmunity due to its key role in induction of Th1 immune responses.

Cytokine detection

IL-12, IL-23 and IL-35 share common subunits, utilizing combinations of p40, p19 and p35 proteins. Caution must be used when selecting antibodies and assays when specific identification, measurement, as well as activation state discrimination, is required.

1. Active IL-12 consists of two subunits: p40 + p35.
2. p40 can also exist as a monomer (IL-12 p40) or a homodimer (IL-12 p80).
3. Besides contributing to IL-12, p40 is also found in IL-23, a heterodimer of p40 and p19.
4. Similarly, p35 not only contributes to IL-12, but is also found in the heterodimer IL-35 (p35 and EBI3).

Note that assays using antibodies specific for the p40 subunit will be unable to discriminate between the active IL-12, monomeric p40, dimeric p40, and IL-23; likewise, assays using p35 detection alone will pick up both IL-12 (heterodimer) and IL-35.

BioLegend ELISA assays

BioLegend’s LEGEND MAX™ Human IL-12 (p70) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-12 (p70) specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human IL-12 (p70) ELISA Kit is specifically designed for the accurate quantitation of Human IL-12 (p70) from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human IL-12 (p70) Pre-coated 96-well Strip Microplate
  • Human IL-12 (p70) Dectection Antibody
  • Human IL-12 (p70) Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Product Citations
  1. Sun J, et al. 2015. J Neuroinflammation. 165:12. PubMed
Sensitivity
1.2 pg/mL
Standard Range
3.9-250 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
3592 View all products for this Gene ID 3593 View all products for this Gene ID
UniProt
View information about IL-12 p70 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 3    Revision Date: 01.27.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account