- Clone
- S21011A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lyt3, Ly3, P37, LEU2, CD8B1, CD8b, CD8beta
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S21011A_PerCP-Cyanine55_-CD8b_Antibody_071823 S21011A_PerCP-Cyanine55_-CD8b_Antibody_071823](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S21011A_PerCP-Cyanine55_-CD8b_Antibody_071823-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD8b (clone S21011A) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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387309 | 25 tests | 100€ | ||||
387310 | 100 tests | 252€ |
CD8b or CD8β antigen is also known as Lyt3, or Ly-3. It is a member of the immunoglobulin superfamily expressed on most thymocytes, and some mature T cell subsets. CD8β forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase LCK (p56lck).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Immunogen
- Human CD8α/CD8β-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Clone S21011A does not interfere with the binding of CD8α binding antibodies (SK1, HIT8a, RPA-T8).
Clone S21011A shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.
Based on in-house testing, clone S21011A clone reacts with an epitope that is exclusively present on human CD8b, whereas clone QA20A40 reacts with an epitope formed by a heterodimer of human CD8a and CD8b chains. - RRID
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AB_3068210 (BioLegend Cat. No. 387309)
AB_3068210 (BioLegend Cat. No. 387310)
Antigen Details
- Structure
- Ig superfamily
- Distribution
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Subset of T Cells, most thymocytes
- Function
- TCR co-receptor
- Interaction
- CD8α
- Ligand/Receptor
- MHC Class 1 molecules
- Cell Type
- Lymphocytes, T cells, Thymocytes
- Biology Area
- Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
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- Johnson P. 1987. Immunogenetics. 26:174-7.
- Norment AM & Littman DR, 1988. EMBO J. 7:3433-9.
- Gene ID
- 926 View all products for this Gene ID
- UniProt
- View information about CD8beta on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD8b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD8b | S21011A | FC |
FITC anti-human CD8b | S21011A | FC |
APC anti-human CD8b | S21011A | FC |
PE anti-human CD8b | S21011A | FC |
PerCP/Cyanine5.5 anti-human CD8b | S21011A | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD8b
Human peripheral blood lymphocytes were stained with purifi... -
FITC anti-human CD8b
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD8b
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD8b
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD8b
Human peripheral blood lymphocytes were stained with anti-hu...
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